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The biotechnology of hydrogen production by Nostoc flagelliforme grown under chemostat conditions

机译：在恒化器条件下生长的鞭毛成虫的制氢生物技术

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摘要

The potential of using N-2-fixing cyanobacteria to produce hydrogen photobiologically has stimulated research on the physiology and biotechnology of species exhibiting high H-2 production rates over long periods of time. In this work Nostoc flagelliforme, a terrestrial N-2-fixing cyanobacterium, has been examined to establish its physiology and potential for H-2 production under controlled conditions. Cell filaments of N. flagelliforme were purified and grown in liquid culture to optimize its H-2 metabolism. In batch-grown cultures the activity of nitrogenase, the key enzyme for H-2 production in N-2-fixing organisms, was found to be high only during a short phase of exponential growth. A chemostat system was thus constructed for long-term experiments using continuous cultures, with the aim of exploiting the exponential growth phase. The dilution rate (D) and environmental factors, such as N-2 concentration in the gas phase and temperature, significantly influenced H-2 production. Cells grown continuously under the optimized conditions of D = 0.022 h(-1), 34 degrees C and 5.1 kPa N-2 in the gas phase exhibited H-2 production rates that were more than four times higher than the maximal rates under standard batch growth conditions.
机译：利用N-2固定蓝细菌以光生物学方式产生氢的潜力激发了长期显示高H-2产生率物种的生理学和生物技术研究。在这项工作中，已经对陆地上的N-2-固定蓝细菌Nostoc鞭毛进行了研究，以确定其生理学和在受控条件下产生H-2的潜力。鞭毛猪笼草的细胞丝被纯化并在液体培养中生长以优化其H-2代谢。在分批生长的培养物中，仅在指数增长的短时期内，发现固氮菌（固氮生物中产生H-2的关键酶）的活性很高。因此，为了利用指数生长期，使用连续培养构建了用于持续实验的化学恒温器系统。稀释率（D）和环境因素（例如气相和温度中的N-2浓度）显着影响了H-2的产生。在D = 0.022 h（-1），34摄氏度和5.1 kPa N-2的最佳条件下在气相中连续生长的细胞表现出的H-2生产率比标准批次的最大生产率高四倍以上生长条件。

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