• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Blood purification >Antioxidant and oxidative stress indices in dialysis-dependent acute renal failure.
【24h】

Antioxidant and oxidative stress indices in dialysis-dependent acute renal failure.

机译：依赖透析的急性肾功能衰竭患者的抗氧化和氧化应激指标。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Experimental animal models and in vitro studies have established a role for reactive oxygen species and the therapeutic potential for free radical scavengers in acute renal failure (ARF). Little is known of the effects of hemodialysis and other clinical variables on antioxidant defenses and oxidative stress among patients with ARF. METHODS: We examined antioxidant defenses and oxidative stress status in 24 patients with ARF requiring hemodialysis (HD). Blood samples were drawn prior to the first dialysis session (baseline), as well as before and after the third and sixth dialysis sessions. At each time point, the following parameters were measured: plasma alpha-tocopherol (vitamin E), plasma glutathione peroxidase (GSH-Px), serum total oxygen radical absorbance capacity (ORAC), plasma thiobarbituric acid reactive substances (TBARS), plasma tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10). Multivariate linear regression analyses were performed to examine clinical/laboratory variables associated with antioxidant/oxidative stress indices. The changes in antioxidant/oxidative stress indices over time after initiation of hemodialysis was evaluated in a subgroup of patients (n = 11) who completed six dialysis sessions. Intradialytic changes in antioxidant/oxidative stress indices and the differential impact of cellulose acetate vs. polysulfone dialyzers were also evaluated. RESULTS: Factors associated with alpha-tocopherol level were serum albumin (301 microg/dl upward arrow for each 1 g/dl upward arrow in albumin) and ORAC (188 microg/dl upward arrow for each 1,000 micromol Trolox Eq/l upward arrow in ORAC). Age was independently associated with plasma GSH-Px levels (55 U/l downward arrow for each 10-year age upward arrow ). Factors associated with ORAC were alpha-tocopherol (85 micromol Trolox Eq/l upward arrow for each 100 microg/dl upward arrow in alpha-tocopherol) and total bilirubin (30 micromol Trolox Eq/l downward arrow for each 1 mg/dl upward arrow in total bilirubin). Total bilirubin was independently associated with TBARS (0.2 microM upward arrow for each 1 mg/dl upward arrow in total bilirubin). GSH-Px and ORAC levels declined over time between baseline and the sixth dialysis session (p < 0.05 for both). Finally, there was a significant intradialytic decline in ORAC levels, which appeared to be more pronounced with use of cellulose acetate compared with polysulfone dialyzer membranes (p < 0.05). CONCLUSIONS: These observations indicate that antioxidant and oxidative stress indices in ARF patients are associated with several clinical and laboratory variables as well as the dialysis procedure. Further studies are needed to investigate the therapeutic role of anti-oxidant therapy in these patients.
机译：背景：实验动物模型和体外研究已经确定了活性氧的种类以及自由基清除剂在急性肾衰竭（ARF）中的治疗潜力。血液透析和其他临床变量对ARF患者的抗氧化防御和氧化应激的影响知之甚少。方法：我们检查了24名需要血液透析（HD）的ARF患者的抗氧化防御和氧化应激状态。在第一次透析之前（基线）以及第三次和第六次透析之前和之后抽取血样。在每个时间点测量以下参数：血浆α-生育酚（维生素E），血浆谷胱甘肽过氧化物酶（GSH-Px），血清总氧自由基吸收能力（ORAC），血浆硫代巴比妥酸反应性物质（TBARS），血浆肿瘤坏死因子-α（TNF-alpha）和白介素10（IL-10）。进行多元线性回归分析以检查与抗氧化剂/氧化应激指数相关的临床/实验室变量。在完成六个透析疗程的亚组患者（n = 11）中，评估了血液透析开始后抗氧化剂/氧化应激指数随时间的变化。还评估了抗氧化剂/氧化应激指数的透析内变化以及醋酸纤维素与聚砜透析器的差异影响。结果：与α-生育酚水平相关的因素包括血清白蛋白（白蛋白中每1 g / dl向上箭头为301 microg / dl向上箭头）和ORAC（每1,000 micromol Trolox Eq / l上箭头为188 microg / dl向上箭头） ORAC）。年龄与血浆GSH-Px水平独立相关（每10岁年龄向上箭头55 U / l向下箭头）。与ORAC相关的因素是α-生育酚（α-生育酚中每100 microg / dl向上箭头为85 micromol Trolox Eq / l向上箭头）和总胆红素（每1 mg / dl向上箭头为30 micromol Trolox Eq / l向下箭头总胆红素）。总胆红素与TBARS独立相关（总胆红素中每1 mg / dl向上箭头为0.2 microM向上箭头）。在基线和第六次透析之间，GSH-Px和ORAC水平随时间下降（两者均p <0.05）。最后，透析中ORAC含量显着下降，与聚砜透析膜相比，使用醋酸纤维素似乎更为明显（p <0.05）。结论：这些观察结果表明，ARF患者的抗氧化和氧化应激指数与一些临床和实验室变量以及透析程序有关。需要进一步的研究来研究抗氧化疗法在这些患者中的治疗作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号