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首页> 外文期刊>Journal of Cellular Physiology >Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells
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Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells

机译：代的神经Crest-Like从人类细胞牙周韧带细胞衍生诱导多能干细胞

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Neural crest cells (NCC) hold great promise for tissue engineering, however the inability to easily obtain large numbers of NCC is a major factor limiting their use in studies of regenerative medicine. Induced pluripotent stem cells (iPSC) are emerging as a novel candidate that could provide an unlimited source of NCC. In the present study, we examined the potential of neural crest tissue-derived periodontal ligament (PDL) iPSC to differentiate into neural crest-like cells (NCLC) relative to iPSC generated from a non-neural crest derived tissue, foreskin fibroblasts (FF). We detected high HNK1 expression during the differentiation of PDL and FF iPSC into NCLC as a marker for enriching for a population of cells with NCC characteristics. We isolated PDL iPSC- and FF iPSC-derived NCLC, which highly expressed HNK1. A high proportion of the HNK1-positive cell populations generated, expressed the MSC markers, whilst very few cells expressed the pluripotency markers or the hematopoietic markers. The PDL and FF HNK1-positive populations gave rise to smooth muscle, neural, glial, osteoblastic and adipocytic like cells and exhibited higher expression of smooth muscle, neural, and glial cell-associated markers than the PDL and FF HNK1-negative populations. Interestingly, the HNK1-positive cells derived from the PDL-iPSC exhibited a greater ability to differentiate into smooth muscle, neural, glial cells and adipocytes, than the HNK1-positive cells derived from the FF-iPSC. Our work suggests that HNK1-enriched NCLC from neural crest tissue-derived iPSC more closely resemble the phenotypic and functional hallmarks of NCC compared to the HNK1-low population and non-neural crest iPSC-derived NCLC. J. Cell. Physiol. 232: 402-416, 2017. (c) 2016 Wiley Periodicals, Inc.
机译：神经嵴细胞(NCC)蕴含着巨大的希望组织工程,但是不能容易获得大量的NCC是主要的因素限制了他们在研究中使用再生医学。细胞(iPSC)正在成为一种新的候选人能提供无限的NCC来源。目前的研究中,我们研究的潜力神经嵴tissue-derived牙周韧带(PDL) iPSC分化成神经相对于iPSC crest-like细胞(NCLC)从非神经嵴派生传播有关组织生成,包皮成纤维细胞(FF)。在PDL的分化和表达式FF iPSC的浓缩成NCLC标记NCC特色的细胞。孤立的PDL iPSC - FF iPSC-derived NCLC,HNK1高度表达。HNK1-positive细胞种群生成,表达了MSC标记,而很少的细胞多能性标记或表示造血标记。HNK1-positive种群产生光滑肌肉、神经胶质,成骨细胞的和adipocytic细胞和更高的展出表达式的平滑肌、神经和神经胶质比PDL细胞相关标记和FFHNK1-negative人群。来源于PDL-iPSC HNK1-positive细胞分化成表现出更大的能力平滑肌、神经胶质细胞和比HNK1-positive脂肪细胞,细胞从FF-iPSC。从神经嵴HNK1-enriched NCLCtissue-derived iPSC更相似表型和功能NCC的标志而HNK1-low人口和非神经嵴传播有关iPSC-derived NCLC。杂志。232:402 - 416年,2017年。期刊、公司。

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