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首页> 外文期刊>Journal of Cellular Physiology >Mouse embryonic stem cells that survive γ-rays exposure maintain pluripotent differentiation potential and genome stability
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Mouse embryonic stem cells that survive γ-rays exposure maintain pluripotent differentiation potential and genome stability

机译：老鼠胚胎干细胞,生存的γ射线接触维持多能分化潜力和基因组的稳定性

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摘要

This study aimed to investigate the cell cycle, apoptosis, cytogenetics and differentiation capacity of mouse embryonic stem cells (mESCs) that survived a single dose of 2 or 5Gy γ-rays during a period of up to 96h of culture. After 2Gy irradiation and 24h culture, compared to control, a significant majority of cells was blocked at the G2/M phase and a massive apoptosis was recorded. Between 48 and 72h post-irradiation, the parameters used to describe the cell cycle and apoptosis returned similar to those of control samples. When mESCs were irradiated with 5Gy, a small fraction of cells, even after 96h of culture, still presented clear evidences of a G2/M block and apoptosis. The cytogenetic analysis performed at 96h showed that the structural stability of the aberrations did not change significantly when comparing control and 2 or 5Gy-treated populations. However, the chromosomal damage observed in the progeny of the survived cells after 5Gy exposure is significantly higher than that observed in control samples, although it is mostly of the stable and transmissible type. Ninety-six hours after irradiation, the survived mESCs maintained their undifferentiated status and capability to differentiate into the three germ layers. Overall, these results indicate a commitment of mESCs to maintain pluripotency and genome stability.
机译：本研究旨在探讨细胞周期,细胞凋亡、细胞遗传学和分化小鼠胚胎干细胞的能力(制)幸存的单剂量2或5 gyγ射线期间96 h的文化。2 gy辐照和24小时文化,相比控制,大部分细胞阻塞在G2 / M期细胞凋亡被记录。机理,用于描述的参数类似于返回的细胞周期和细胞凋亡控制样品。辐照与5 gy,一小部分细胞,即使96 h的文化,还介绍清楚证据的G2 / M块和细胞凋亡。在96 h表明,细胞遗传学分析的结构稳定性畸变当比较控制改变不明显和2或5 gy-treated人群。染色体损伤的后代细胞5 gy曝光后幸存下来明显高于观察控制样本,尽管它是主要的稳定和传播类型。制维护辐照后,幸存下来他们的未分化的状态和能力分化成三个胚芽层。整体而言,这些结果表明的承诺制维持多能性和基因组稳定。

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