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Study of the genomic constitution of the German mirror carp

机译：德国镜鲤的基因组学研究

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Semen was treated with ultraviolet irradiation to genetically inactivate the sperm. Gynogenetic haploidy was induced artificially in the German mirror carp, Cyprinus carpio L. A systematic study of the status of early embryonic development, the number of chromosomes, chromosomal banding patterns and fluorescent in situ hybridization (FISH), and a microsatellite analysis were performed. The fertility, hatchability, and survival rates of the gynogenetic group were significantly lower than those of the normal control fertilization group (P 0.05). Gynogenetic larval growth was abnormal and the embryos did not survive. The chromosome number of the gynogenetic group was 50, whereas that of the control group was 100. The gynogenetic group had a single silver-staining point, one CMA(3)/DA/DAPI-positive position, and a single FISH-positive signal, whereas the control group had two silver-staining points, two CMA(3)/DA/DAPI-staining positions, and two FISH-positive signals. The microsatellite analysis showed that all the genes of the gynogenetic group derived from the female parent and that the females generated a set of haploid (n = 50) gametes during meiosis. Using chromosome manipulation, this study confirms that the German mirror carp has two sets of chromosomes and is an evolutionary tetraploid (2n = 100).
机译：用紫外线照射精液使精子遗传失活。人工生殖单倍体是在德国镜鲤（Cyprinus carpio L）中人工诱导的。系统研究了早期胚胎发育的状况，染色体数，染色体条带模式和荧光原位杂交（FISH），并进行了微卫星分析。雌激素发育组的生育率，孵化率和成活率均明显低于正常对照受精组（P <0.05）。雌核发育的幼虫生长异常，胚胎无法存活。雌成虫组的染色体数为50，而对照组为100。该雌成虫组具有一个银染点，一个CMA（3）/ DA / DAPI阳性位置和一个FISH阳性信号。 ，而对照组有两个银染点，两个CMA（3）/ DA / DAPI染点和两个FISH阳性信号。微卫星分析表明，雌雄同体发育组的所有基因均来自雌性亲本，雌性在减数分裂过程中产生了一组单倍体（n = 50）配子。使用染色体操纵，这项研究证实了德国镜鲤具有两组染色体，并且是进化四倍体（2n = 100）。

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