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首页> 外文期刊>Journal of Cellular Physiology >Notch2 negatively regulates myofibroblastic differentiation of myoblasts.
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Notch2 negatively regulates myofibroblastic differentiation of myoblasts.

机译：Notch2负调节染色法成肌细胞的分化。

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摘要

Myofibroblasts are one of the key cellular components involved in fibrosis of skeletal muscle as well as in other tissues. Transforming growth factor-beta1 (TGF-beta1) stimulates differentiation of mesenchymal cells into myofibroblasts, but little is known about the regulatory mechanisms of myofibroblastic differentiation. Since Notch2 was shown to be downregulated in TGF-beta1-induced non-muscle fibrogenic tissue, we investigated whether Notch2 also has a distinctive role in myofibroblastic differentiation of myogenic cells induced by TGF-beta1. TGF-beta1 treatment of C2C12 myoblasts led to expression of myofibroblastic marker alpha-smooth muscle actin (alpha-SMA) and collagen I with concomitant downregulation of Notch2 expression. Overexpression of active Notch2 inhibited TGF-beta1-induced expression of alpha-SMA and collagen I. Interestingly, transient knockdown of Notch2 by siRNA in C2C12 myoblasts and primary cultured muscle-derived progenitor cells resulted in differentiation into myofibroblasticcells expressing alpha-SMA and collagen I without TGF-beta1 treatment. Furthermore, we found Notch3 was counter-regulated by Notch2 in C2C12 cells. These findings suggest that Notch2 is inhibiting differentiation of myoblasts into myofibroblasts with downregulation of Notch3 expression.
机译：Myofibroblasts是一个关键的细胞组件参与肝纤维化的骨骼肌肉和其他组织。增长factor-beta1 (TGF-beta1)刺激间充质细胞的分化myofibroblasts,但很少知道监管机制的染色法分化。表达下调在TGF-beta1-induced non-muscle纤维发生的组织,我们调查是否Notch2在染色也有独特的作用肌原性的细胞分化诱导TGF-beta1。导致染色法标记的表达alpha-smooth肌肉肌动蛋白(alpha-SMA)和胶原蛋白的差别我和伴随的对这些Notch2表达式。Notch2抑制TGF-beta1-induced表达alpha-SMA和胶原蛋白。有趣的是,瞬态的Notch2击倒在C2C12核成肌细胞和主要培养肌源性祖细胞分化成myofibroblasticcells表达alpha-SMA和胶原蛋白我没有TGF-beta1治疗。此外,我们发现Notch3counter-regulated Notch2在C2C12细胞。研究结果表明,Notch2抑制成肌细胞分化成myofibroblastsNotch3的差别,对这些基因的表达。

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