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首页> 外文期刊>Journal of Cellular Physiology >Regulation of Puralpha gene transcription: evidence for autoregulation of Puralpha promoter.
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Regulation of Puralpha gene transcription: evidence for autoregulation of Puralpha promoter.

机译：Puralpha基因转录的调节:证据Puralpha启动子的自动调整。

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The single-stranded DNA and RNA binding protein, Puralpha, has recently received special attention as this protein, by associating with the specific nucleotide sequence (GGN repeats) and/or several important cellular and viral proteins regulates crucial biological events such as transcription, replication, and cell proliferation. In this study, we focused on the promoter activity of the Puralpha upstream DNA sequence and demonstrated that the sequence spanning 6,000 nucleotides upstream of the Puralpha transcription start site has promoter activity in various cell types. Results from promoter deletion studies revealed that this region encompasses various regulatory motifs which differentially participate in the promoter activity of Puralpha in various cells. The transcription start site of Puralpha is surrounded by the GA/GC-rich sequence which exhibits the ability to interact with Puralpha, suggesting a role for autoregulation of Puralpha transcription. Results from co-transfection studies revealed that ectopic expression of Puralpha reduced transcriptional activity of the Puralpha promoter and the region located between amino acid residues, 1-85 of Puralpha is important for the observed autoregulatory event. The regulatory protein of the human neurotropic virus, JCV, T-antigen, which interacts with Puralpha, decreased transcriptional activity of the Puralpha promoter. Co-expression of JCV T-antigen and Puralpha had no significant effect on the suppression of Puralpha gene transcription by either protein. The importance of this finding in light of earlier results showing down regulation of Puralpha during JCV infection of glial cells is discussed. Copyright 2001 Wiley-Liss, Inc.
机译：单链DNA和RNA结合蛋白,Puralpha,最近受到了特殊的关注这种蛋白质,与具体相关联核苷酸序列(GGN重复)和/或几个重要的细胞和病毒蛋白调节至关重要的生物事件,如转录,复制和细胞增殖。研究中,我们关注的推广活动Puralpha上游DNA序列,并演示了跨越6000个核苷酸序列上游的Puralpha转录起始站点具有启动子活性在不同的细胞类型。从启动子缺失的研究显示结果这个地区包括各种监管参与不同的主题启动子的活性Puralpha在各种细胞。转录起始站点PuralphaGA / GC-rich序列包围展品与Puralpha交互的能力,Puralpha自动调整的暗示作用转录。研究表明,异位表达的Puralpha转录活动的减少Puralpha启动子和该地区位于两者之间氨基酸残基,Puralpha 1 - 85对观察到的自身调节的重要事件。监管人类亲神经的的蛋白质与病毒、JCV t抗原Puralpha,转录活性的下降Puralpha启动子。t抗原和Puralpha没有显著的影响Puralpha的抑制基因转录通过蛋白质。在早些时候的结果显示监管期间Puralpha JCV感染神经胶质细胞进行了探讨。Wiley-Liss公司。

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