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Dietary L-lysine requirement of juvenile Chinese sucker, Myxocyprinus asiaticus

机译：少年中国吸盘的日粮赖氨酸需求量

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An 8-week feeding trial was conducted to quantify the dietary l-lysine requirement of juvenile Chinese sucker with an initial weight of 1.81 +/- 0.04g reared in indoor flow-through and aerated tanks. Six isonitrogenous and isoenergetic practical diets were formulated to contain graded levels of lysine (1.23%, 1.80%, 2.39%, 2.98%, 3.56% and 4.18% dry matter) at 0.6% increments from dietary ingredients and crystalline l-lysine. Each diet was randomly assigned to triplicate groups of 30 fish each and was fed to apparent satiation by hand three times a day (09:00, 13:00 and 17:00hours) for 8weeks. There were significant differences in growth performance and feed utilization among the treatments. Weight gain (WG), specific growth rate and protein efficiency ratio (PER) significantly increased with increasing lysine levels up to 2.39% of diet (P<0.05) and remained nearly the same thereafter (P>0.05). Feed efficiency was the poorest for fish fed the lowest lysine diet (P<0.05) and showed no significant differences when dietary lysine level increased from 2.39% to 4.18%. The N retention (% N intake) significantly increased with dietary lysine level but did not attain a plateau (P<0.05). Survival could not be related to dietary treatments. Whole body protein increased (P<0.05) and whole body lipid decreased (P<0.05) with increasing dietary lysine level. The condition factor and hepatosomatic index were significantly affected by dietary lysine levels, however, viscersomatic index, whole body moisture and ash did not differ significantly among dietary treatments. Broken-line analysis on the basis of WG and PER showed that dietary lysine requirements of juvenile Chinese sucker were 2.43% and 2.40% dry diet (5.52% and 5.45% dietary protein) respectively. Based on the ideal protein approach and the A/E ratios determined from muscle amino acid profile an estimation of the EAA requirements of Chinese sucker juveniles were calculated.
机译：进行了一个为期8周的喂养试验，以定量定量在室内流通池和充气池中饲养的初生中国吸盘的日粮赖氨酸需求量，初始重量为1.81 +/- 0.04g。配制了六种等氮和等能量的实用日粮，其中日粮成分和结晶的左赖氨酸含量分别为0.6％的赖氨酸（1.23％，1.80％，2.39％，2.98％，3.56％和4.18％干物质）。每种饮食被随机分为三组，每组30条鱼，并每天人工喂饱3周（09：00、13：00和17:00小时），持续8周。各处理之间的生长性能和饲料利用率存在显着差异。随着赖氨酸水平的增加，体重增加（WG），比生长速率和蛋白质效率比（PER）显着增加，达到饮食的2.39％（P <0.05），此后几乎保持不变（P> 0.05）。饲喂最低赖氨酸饮食的鱼的饲料效率最差（P <0.05），并且当赖氨酸水平从2.39％增加到4.18％时，饲料效率没有显着差异。氮的保留（氮的摄入百分比）随着饮食中赖氨酸水平的增加而显着增加，但未达到平稳状态（P <0.05）。生存与饮食疗法无关。随着饮食中赖氨酸水平的升高，全身蛋白质增加（P <0.05），而全身脂质减少（P <0.05）。饮食赖氨酸水平显着影响条件因子和肝体指数，但是饮食治疗之间的内脏指数，全身水分和灰分没有显着差异。根据WG和PER进行的折线分析显示，青少年中国吸盘的日粮赖氨酸需求量分别为干饮食的2.43％和2.40％（饮食蛋白质的5.52％和5.45％）。基于理想的蛋白质方法和由肌肉氨基酸谱确定的A / E比，计算出中国吸盘少年的EAA需求量的估算值。

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