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首页> 外文期刊>Journal of Cellular Physiology >Possible involvement of p21 but not of p16 or p53 in keratinocyte senescence.
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Possible involvement of p21 but not of p16 or p53 in keratinocyte senescence.

机译：可能参与p21但不是p16和p53在角化细胞衰老。

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It has been reported that p21, p53, and p16 affect the cell cycle and cell senescence. However, their roles in keratinocyte senescence are not clear. We established primary keratinocyte strains from 15 donors and maintained them until replicative senescence; their population doublings ranged from 5.7-45.2. These strains were classified based on their population doublings as short (5.7-10.4), intermediate (13.9-17.4), and long (21.5-45.2). To investigate the roles of p21, p53, and p16 in the cellular senescence of the cultured keratinocytes, we quantitatively analyzed p21, p53, and p16 levels of keratinocyte strains with different life spans by Western blot with Fluorol mager. p21 levels increased in the senescent phase but not in the nonsenescent phase in all of the short, intermediate, and long life-span strains. Northern blot analysis also revealed induction of p21 mRNA was similar to that of p21 protein levels. There were no apparent differences in p53 levels between senescent and nonsenescent cells. The short life-span strains exhibited a significant increase in p16 levels in the senescent phase (eighth or tenth passage). However, in two long life-span strains, p16 levels were increased in the nonsenescent phase (eighth passage) but then declined as the cells reached senescence (twenty-seventh passage). Therefore, induction of p16 appeared not to be associated with senescence in long life-span strains. In conclusion, p21 but not p16 or p53 may play roles in keratinocyte senescence.
机译：据报道,p21、p53和p16影响细胞周期和细胞衰老。他们的角色在角化细胞衰老明确的。直到压力来自15个捐助者和维护复制衰老;范围从5.7 - -45.2倍增。分类基于他们的人口短倍增(5.7 - -10.4),中间(13.9 - -17.4),长(21.5 - -45.2)。p21的角色、p53和p16在细胞培养的角质细胞的衰老,我们定量分析了p21、p53和p16的水平角化细胞的菌株有不同的寿命通过免疫印迹Fluorol瘦的。在衰老阶段而不是增加在所有的短nonsenescent阶段,中间,长寿命菌株。北部污点分析还揭示了感应的p21 mRNA p21蛋白相似的水平。衰老和nonsenescent细胞之间的水平。短寿命菌株表现出一个p16水平大幅提高衰老阶段(八或十通道)。然而,在两个长寿命菌株,p16水平增加nonsenescent阶段(第八段)然后拒绝的细胞达到衰老(二十七通道)。因此,感应p16不出现与衰老相关的长寿命菌株。可能在角化细胞衰老中扮演角色。

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