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首页> 外文期刊>Aquaculture Research >The effect of individual male potency on fertilization ability of freshand cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum)
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The effect of individual male potency on fertilization ability of freshand cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum)

机译：雄性个体效力对虹鳟Oncorhynchus mykiss（Walbaum）新鲜和低温保存的虹鳟受精能力的影响

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摘要

The goal of the present study was to examine individual male potency in rainbow trout, Oncorhynchus mykiss (Walbaum), expressed as the fertilization ability of fresh and cryopreserved sperm. One female and four males bearing genetic markers enabling determination of the progeny paternity were chosen as gamete donors. Samples of eggs were inseminated with sperm from separate individuals or with pooled sperm. Genetic examination of the progeny obtained after fertilization of eggs with pooled milt showed differences in male potency. The proportions of offspring sired by four individual males after fertilization of eggs with the fresh milt were similar to those obtained after fertilization with cryopreserved milt (correlation r = 0.95; n = 4: P < 0.05). These proportions did not correlate with the proportions of progeny resulting from fertilization of eggs when sperm was not pooled.
机译：本研究的目的是检查虹鳟鳟鱼（Walbaum）中的男性雄性能力，以新鲜和冷冻精子的受精能力表示。选择具有遗传标志物的一女和四雄来确定子代亲本，作为配子供体。卵的样本用不同个体的精子或合并的精子进行授精。卵子混合后对卵子受精后的后代进行了基因检查，结果显示雄性效力存在差异。用新鲜的麦芽对卵进行受精后，由四个单独的雄性所生的子代比例与冷冻保存的麦芽对受精后的比例相似（相关系数r = 0.95; n = 4：P <0.05）。这些比例与不合并精子时卵受精产生的后代比例不相关。

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