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首页> 外文期刊>Quality Assurance and Safety of Crops & Foods >Development of multiplex-PCR systems for genes related to flour colour in Chinese autumn-sown wheat cultivars
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Development of multiplex-PCR systems for genes related to flour colour in Chinese autumn-sown wheat cultivars

机译：复合pcr系统发展的基因有关中国autumn-sown面粉颜色小麦品种

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In wheat kernel, multiple gene loci, Psy-A1, Psy-B1, TaZds-A1, TaZds-D1, TaLox-B1, Ppo-A1 and Ppo-D1 are implicated in the expression of flour colour traits. In this study, eight functional markers, covering seven gene loci, were used to develop three multiplexed polymerase chain reaction (PCR) systems for marker-assisted selection (MAS) of desirable flour colour, i.e. PCR-I (YP7B-1/YP7B-2/YP2A-1), PCR-II (YP2D-1/PPO18) and PCR-III (YP7A/LOX18/STS01). Genotyping of 20 control wheat cultivars by these multiplex-PCR systems completely matched the results from the eight single PCR amplifications. Multiplex-PCR typing was successfully established for a total of 155 Chinese autumn-sown wheat cultivars. In multiplexed PCR-I, the occurring frequencies for three alleles (Psy-B1a, Psy-B1b and Psy-B1c) at the Psy-B1 locus were 33.5, 58.7, and 7.7%; while two alleles (TaZds-A1a and TaZds-A1b) at TaZds-A1 were 40.0 and 60.0%, respectively. In multiplexed PCR-II, the occurring frequencies, TaZds-D1a and TaZds-D1b (98.7 and 1.3%) at the TaZds-D1 locus, Ppo-A1a and Ppo-A1b (39.4 and 60.6%) at the Ppo-A1 locus were obtained, respectively. In multiplexed PCR-III, the occurring frequencies for two predominant alleles at each locus was obtained, Psy-A1a and Psy-A1b (55.5 and 44.5%) at Psy-A1 locus, TaLox-B1a and TaLox-B1b (29.7 and 70.3%) at TaLox-B1 locus, Ppo-D1a and Ppo-D1b (45.8 and 54.2%) at Ppo-D1 locus, respectively. The multiplexed PCRs developed in this study are useful for MAS for wheat flour colour improvement. The genotyping results obtained in this study provide valuable information for DNA-based identification of wheat cultivars.
机译：在小麦的内核,多个基因位点、Psy-A1Psy-B1、TaZds-A1 TaZds-D1、TaLox-B1 Ppo-A1和Ppo-D1与面粉的表达颜色特征。标记,包括7个基因位点,被用来开发三个多路复用聚合酶链式反应(PCR)对分子标记辅助系统选择(MAS)的面粉颜色,即。(YP2D-1 / PPO18)和PCR-III (YP7A / LOX18 / STS01)。基因分型20通过这些控制小麦品种复合pcr系统完全匹配结果8单一PCR扩增。复合pcr输入被成功建立总共155名中国autumn-sown小麦品种。三个等位基因频率(Psy-B1a Psy-B1b和Psy-B1c) Psy-B1轨迹分别为33.5,58.7,和7.7%;TaZds-A1 TaZds-A1b)分别为40.0和60.0%,分别。发生频率,TaZds-D1a TaZds-D1b(1.3%和98.7)TaZds-D1轨迹,Ppo-A1a和Ppo-A1b Ppo-A1轨迹(60.6%和39.4)分别获得。PCR-III,发生的频率占主导地位的获得在每个位点的等位基因,Psy-A1a和Psy-A1b Psy-A1(44.5%和55.5)轨迹,TaLox-B1a TaLox-B1b(70.3%和29.7)在TaLox-B1轨迹,Ppo-D1a和Ppo-D1b (45.8分别为54.2%)在Ppo-D1轨迹。多路复用pcr在本研究开发有用的MAS的小麦面粉的颜色改进。这项研究提供有价值的信息dna鉴定小麦品种。

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