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首页> 外文期刊>Applied radiation and isotopes: including data, instrumentation and methods for use in agriculture, industry and medicine >USE OF INAA TO STUDY THE DETERMINATION OF SE, TH, ZN, CO AND FE LEVELS OF YEAST CELLS
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USE OF INAA TO STUDY THE DETERMINATION OF SE, TH, ZN, CO AND FE LEVELS OF YEAST CELLS

机译：使用INAA研究测定酵母细胞中的SE，TH，ZN，CO和FE水平

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摘要

Differences in the effects of seleno-cystine (CySe)(2), glutathione (GSH), Se(IV) [as SeO2] and Se(VI) [as (NH4)(2)SeO4] on Th(IV) [as Th(CO3)(2)] uptake by the cells, Saccharomyces cerevisiae, have been studied. The Th, Se, Zn, Co and Fe levels of the yeast cells were measured by instrumental neutron activation analysis. Results obtained show that the addition of Th alone to the culture medium resulting in the Th content of the cells and the Th level of the yeast slightly decreased during the incubation. The addition of Th in combination with GSH produced a higher decrease of the Th content in comparison with the single Th dosage. During the initial 48 h of the incubation the presence of Th and Se(VI) in the medium produced a decrease of the Th level of the cells in comparison with the addition of Th alone. (CySe)(2) or SeO2 does not produce a regular change of the Th level of the cells. Th uptake by the yeast influenced the retention of Sein the cells. In fact, the Se levels of the cells were always significantly higher when the yeast was incubated in the medium containing Th and SeO2 or Se(VI). The enhance in the Se level of the cells rises with increasing concentrations of SeO2 in the culture medium. Th decreased the Se content of the yeast when the cells were incubated in the medium containing (CySe)(2) and Th. GSH supply in combination with Th and SeO2 produced a very significant enhancement of the Se abundance in the cells in comparison with the single addition of SeO2. Se-compounds and/or Th dosages affected the Zn, Co and Fe contents of the cells. The Fe level of the yeast is below the quantitative detection limit of Fe when the cells were incubated in the medium containing Th.
机译：硒代半胱氨酸（CySe）（2），谷胱甘肽（GSH），Se（IV）[as SeO2]和Se（VI）[as（NH4）（2）SeO4]对Th（IV）作用的差异已经研究了酿酒酵母对Th（CO3）（2）的吸收。通过仪器中子活化分析测量酵母细胞的Th，Se，Zn，Co和Fe水平。获得的结果表明，在培养过程中，单独向培养基中添加Th导致细胞中Th含量和酵母Th含量略有下降。与单一Th剂量相比，将Th与GSH结合使用会使Th含量降低更多。在孵育的最初48小时内，与单独添加Th相比，培养基中Th和Se（VI）的存在降低了细胞Th的水平。 （CySe）（2）或SeO2不会产生细胞Th水平的规则变化。酵母的Th摄取影响了Sein细胞的保留。实际上，当酵母在含有Th和SeO2或Se（VI）的培养基中孵育时，细胞的Se水平始终显着较高。随着培养基中SeO 2浓度的增加，细胞中硒水平的提高也随之增加。当在含有（CySe）（2）和Th的培养基中孵育细胞时，Th会降低酵母的Se含量。与仅添加SeO2相比，GSH供应与Th和SeO2的结合显着提高了细胞中硒的丰度。硒化合物和/或Th的剂量影响细胞中锌，钴和铁的含量。当将细胞在含有Th的培养基中孵育时，酵母的Fe水平低于Fe的定量检测极限。

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