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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Epstein-Barr virus (EBV)-induced long-term proliferation of CD4+ lymphocytes leading to T lymphoblastoid cell lines carrying EBV.
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Epstein-Barr virus (EBV)-induced long-term proliferation of CD4+ lymphocytes leading to T lymphoblastoid cell lines carrying EBV.

机译：爱泼斯坦巴尔病毒（EBV）诱导的CD4 +淋巴细胞长期增殖，导致携带EBV的T淋巴母细胞系。

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We have previously demonstrated that Epstein-Barr virus (EBV) strain B95.8 can infect and initiate a partial transcriptional program in both CD4+ and CD8+ lymphocytes (Guan, M. X., R.-D. Zhang, B. Wu, and E. E. Henderson. 1996. J. Virol. 70:7341-7346). Experiments were undertaken to determine whether EBV infection can alter the growth potential of T lymphocytes. Peripheral blood lymphocytes were separated into populations consisting of 99.8% CD4+ and 98.6% CD8+ T lymphocytes by FACS. Infection of these populations with EBV resulted in blastogenesis in both CD4+ and CD8+ populations. Clones were established from the CD4+ population in the presence of interleukin-2. Two of these clones expressed the T cell surface markers CD3 and CD4 and carried the EBV genome. One lymphoblastoid cell line (LCL) had a mixture of CD4+ and CD19+ cells. The T-LCLs harboring the EBV genome in circular form and transcribed mRNA transcripts corresponding to BZLF1, BRLF1, BMLF1, and EBER-1 and -2. Immunofluorescence demonstrated EBNA in the nucleus of T rosette-positive lymphoblasts with an absence of viral capsid antigen expression. In situ hybridization for EBER showed nuclear and cytoplasmic staining in B95.8 cells, whereas EBV-carrying T-LCLs only showed nuclear staining. These results demonstrate that EBV can both infect and induce growth transformation of T lymphocytes, supporting a direct role for EBV in AIDS-related, EBV-associated T cell lymphomas. A better understanding of the EBV transcriptional program during EBV-induced T cell transformation could directly lead to adoptive T cell therapeutic strategies and/or more effective antiviral chemotherapy for EBV-associated T cell lymphoma.
机译：我们以前已经证明了爱泼斯坦巴尔病毒（EBV）株B95.8可以感染CD4 +和CD8 +淋巴细胞并启动部分转录程序（Guan，MX，R.-D.Zhang，B.Wu和EE Henderson。 1996.J.Virol.70：7341-7346）。进行实验以确定EBV感染是否可以改变T淋巴细胞的生长潜力。通过FACS将外周血淋巴细胞分为由99.8％CD4 +和98.6％CD8 + T淋巴细胞组成的群体。这些人群感染EBV导致CD4 +和CD8 +人群都发生了成胚作用。在白介素2存在下从CD4 +群体建立克隆。这些克隆中有两个表达T细胞表面标记CD3和CD4，并带有EBV基因组。一种淋巴母细胞系（LCL）具有CD4 +和CD19 +细胞的混合物。 T-LCL以圆形形式携带EBV基因组，并转录对应于BZLF1，BRLF1，BMLF1和EBER-1和-2的mRNA转录本。免疫荧光显示EB阳性的T花环阳性淋巴母细胞核中没有病毒衣壳抗原表达。 EBER的原位杂交在B95.8细胞中显示出核和细胞质染色，而携带EBV的T-LCL仅显示出核染色。这些结果表明，EBV可以感染并诱导T淋巴细胞的生长转化，从而支持EBV在与AIDS有关的，与EBV相关的T细胞淋巴瘤中的直接作用。在EBV诱导的T细胞转化过程中对EBV转录程序的更好理解可以直接导致针对EBV相关T细胞淋巴瘤的过继性T细胞治疗策略和/或更有效的抗病毒化疗。

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