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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Magnetic resonance and fluorescence-protein imaging of the anti-angiogenic and anti-tumor efficacy of selenium in an orthotopic model of human colon cancer.
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Magnetic resonance and fluorescence-protein imaging of the anti-angiogenic and anti-tumor efficacy of selenium in an orthotopic model of human colon cancer.

机译：硒在人结肠癌原位模型中的抗血管生成和抗肿瘤功效的磁共振和荧光蛋白成像。

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Tumor progression and angiogenesis are intimately related. To understand the interrelationship between these two processes, real-time imaging can make a major contribution. In this report, fluorescent protein imaging (FPI) and magnetic resonance imaging (MRI) were utilized to demonstrate the effects of selenium on tumor progression and angiogenesis in an orthotopic model of human colon cancer. GEO (well-differentiated human colon carcinoma) cells transfected with green fluorescent protein (GFP) were implanted orthotopically into the colon of athymic nude mice. Beginning at five days post implantation, whole-body FPI was performed to monitor tumor growth in vivo. Upon successful visualization of tumor growth by FPI, animals were randomly assigned to either a control group or a treatment group. Treatment consisted of daily oral administration of the organoselenium compound, methyl-selenocysteine (MSC; 0.2 mg/day x five weeks). Dynamic contrast-enhanced MRI was performed to examine the change in tumor blood volume following treatment. CD31 immunostaining of tumor sections was also performed to quantify microvessel density (MVD). While T1- and T2-weighted MRI provided adequate contrast and volumetric assessment of GEO tumor growth, GFP imaging allowed for high-throughput visualization of tumor progression in vivo. Selenium treatment resulted in a significant reduction in blood volume and microvessel density of GEO tumors. A significant inhibition of tumor growth was also observed in selenium-treated animals compared to untreated control animals. Together, these results highlight the usefulness of multimodal imaging approaches to demonstrate antitumor and anti-angiogenesis efficacy and the promise of selenium treatment of colon cancer.
机译：肿瘤的进展与血管生成密切相关。为了了解这两个过程之间的相互关系，实时成像可以做出重大贡献。在此报告中，荧光蛋白成像（FPI）和磁共振成像（MRI）用于证明硒对人结肠癌原位模型中肿瘤进展和血管生成的影响。将绿色荧光蛋白（GFP）转染的GEO（高分化人结肠癌）细胞原位植入无胸腺裸鼠结肠中。从植入后五天开始，进行全身FPI监测体内肿瘤的生长。通过FPI成功观察到肿瘤的生长后，将动物随机分为对照组或治疗组。治疗包括每日口服有机硒化合物甲基硒代半胱氨酸（MSC; 0.2 mg / day x 5周）。进行动态对比增强MRI检查治疗后肿瘤血容量的变化。还对肿瘤切片进行了CD31免疫染色，以定量微血管密度（MVD）。尽管T1和T2加权MRI提供了足够的对比度和对GEO肿瘤生长的体积评估，但GFP成像可实现体内肿瘤进展的高通量可视化。硒治疗导致GEO肿瘤的血容量和微血管密度显着降低。与未处理的对照动物相比，在用硒处理的动物中也观察到对肿瘤生长的显着抑制。总之，这些结果凸显了多峰成像方法对证明抗肿瘤和抗血管生成功效的有效性以及硒治疗结肠癌的前景。

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