Structural and enzymatic characterization of a host-specificity determinant from Salmonella

KohlerA.C.; SpanòS.; GalánJ.E.StebbinsC.E.

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Structural and enzymatic characterization of a host-specificity determinant from Salmonella

机译：结构和酶的特性微型行列式的沙门氏菌

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GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 ? resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections.

机译：GtgE沙门氏菌是一种效应蛋白沙门氏菌感染,调节贩卖的Salmonella-containing液泡。函数通过裂开Rab-family gtpaseRab29, Rab32 Rab38,从而阻止了交付的抗菌因子bacteria-containing液泡。在1.65 GtgE结构吗?,并给出了基于结构突变和分析用来确定其体内感染催化三和弦。抑制剂进行确定N-ethylmaleimide, antipain chymostatin在体外抑制GtgE活动。小说的发展提供了依据治疗策略应对沙门氏菌感染。

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来源
《Acta crystallographica.Section D Biological crystallography.》 |2014年第2期|384-391|共8页
作者
KohlerA.C.; SpanòS.; GalánJ.E.StebbinsC.E.;
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作者单位

Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536;

Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10065, United States;

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正文语种英语
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关键词
BASES(STRUCTURES); Salmonella typhi; cysteine proteinase inhibitorCysteine ProteasesSalmonellarab GTP-Binding ProteinsVacuolesSalmonella typhimuriumchymostatinSalmonella Infections;

机译：ProteasesSalmonellarab GTP-BindingProteinsVacuolesSalmonella;

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Structural and enzymatic characterization of a host-specificity determinant from Salmonella

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