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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >A rapid HPLC method for the measurement of ultra-low plasma methionine concentrations applicable to methionine depletion therapy.
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A rapid HPLC method for the measurement of ultra-low plasma methionine concentrations applicable to methionine depletion therapy.

机译：快速高效液相色谱法，用于测定超低血浆蛋氨酸浓度，适用于蛋氨酸消耗疗法。

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摘要

A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) method for the separation and quantification of L-methionine in plasma has been developed. After derivatization of plasma amino acids with o-phthalaldehyde (OPA), a 50 microl sample was loaded on a reversed-phase Supelcosil LC-18-DB column (particle size 5 microm, 25 cm x 4.6 mm, 120A pores). A customized gradient program using tetrahydrofuran/methanol/0.1 M sodium acetate, pH 7.0, v/v/v=5/95/900 and methanol was used with detection by fluorescence. The elution time was 15 minutes, a 3-fold improvement over existing methods. The linearity was 1-100 microM. The limit of detection was 0.5 micromol/L, a 10-fold improvement over existing methods. The inter-assay CVs were 2-5%, and the intra-assays CVs were 4-8%. The sensitivity and rapidity of this HPLC method is particularly applicable to determine the efficacy of methionine depletion therapy of cancer.
机译：建立了一种快速，灵敏，高效的液相色谱（HPLC）方法，用于血浆中L-蛋氨酸的分离和定量。用邻苯二甲醛（OPA）衍生化血浆氨基酸后，将50微升样品装入反相Supelcosil LC-18-DB色谱柱（粒径5微米，25 cm x 4.6 mm，120A孔）。使用使用四氢呋喃/甲醇/0.1 M乙酸钠，pH 7.0，v / v / v = 5/95/900和甲醇的定制梯度程序，并通过荧光进行检测。洗脱时间为15分钟，比现有方法提高了3倍。线性为1-100 microM。检测限为0.5 micromol / L，比现有方法提高了10倍。批间CV为2-5％，批内CV为4-8％。这种HPLC方法的灵敏性和快速性特别适用于确定蛋氨酸消耗疗法对癌症的疗效。

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