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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Protein expression and gene copy number analysis of topoisomerase 2alpha, HER2 and P53 in minimally invasive urothelial carcinoma of the urinary bladder--a multitissue array study with prognostic implications.
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Protein expression and gene copy number analysis of topoisomerase 2alpha, HER2 and P53 in minimally invasive urothelial carcinoma of the urinary bladder--a multitissue array study with prognostic implications.

机译：拓扑异构酶2α，HER2和P53在膀胱微创性尿路上皮癌中的蛋白质表达和基因拷贝数分析-一种具有预后意义的多组织阵列研究。

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BACKGROUND: Due to their variable clinical course, there is a need for new prognostic parameters in minimally invasive (stage T1) bladder carcinomas. MATERIALS AND METHODS: Using multitissue arrays, protein expression and gene copy numbers of topoisomerase (TOP2alpha), HER2 and p53 were investigated by immunohistochemistry and by fluorescence in situ hybridization (FISH) in 73 T1 tumors. The results were compared with tumor recurrence and progression. RESULTS: The median TOP2alpha and p53 index was 21% (range, 3-59%) and 7% (range, 0-93%), respectively. HER2 overexpression (score 3+) was detected in 9 cases (12%). High TOP2alpha and p53 indices and HER2 overexpression were significantly associated with earlier tumor recurrence, but not with earlier tumor progression. While TOP2alpha and p53 gene amplification was detected in no case, 5 cases (8%) showed HER2 gene amplification, which was related to HER2 3+ score in 4 cases. Loss of TOP2alpha, HER2 and p53 gene was observed in 4 (8%), 8 (13%) and 6 cases (12%), respectively. By univariate analysis, TOP2alpha index (p=0.0267), HER2 score (p =0.028) and p53 index (p=0.0188) were significantly and loss of TOP2alpha gene (p=0.0575) tendentially correlated with tumor recurrence, while loss of HER2 gene (p=0.069) and loss of p53 gene (p=0.0587) were tendentially correlated with tumor progression. In a multivariate analysis, which also included tumor grade and T1 substage, TOP2alpha index (p=0.043) and p53 index (p=0.02) were identified as independent predictors of tumor recurrence and loss of p53 gene (p=0.012) and T1 substage (p=0.029) as independent predictors of tumor progression. CONCLUSION: Immuno-histochemical TOP2alpha and p53 staining as well as FISH analysis of p53 gene copy numbers and T1 substaging are helpful means of providing additional information on the biological behavior of T1 transitional cell carcinomas.
机译：背景：由于其可变的临床过程，因此需要在微创（T1期）膀胱癌中使用新的预后参数。材料与方法：采用免疫组织化学和荧光原位杂交技术（FISH），通过免疫组织化学和荧光原位杂交（FISH）研究了73个T1肿瘤中拓扑异构酶（TOP2α），HER2和p53的蛋白表达和基因拷贝数。将结果与肿瘤复发和进展进行比较。结果：TOP2alpha和p53指数中位数分别为21％（范围3-59％）和7％（范围0-93％）。在9例（12％）中检测到HER2过表达（得分3+）。高TOP2alpha和p53指数以及HER2过表达与较早的肿瘤复发显着相关，但与较早的肿瘤进展无明显关系。虽然未检测到TOP2alpha和p53基因扩增，但5例（8％）显示HER2基因扩增，这与4例中HER2 3+得分有关。分别在4例（8％），8例（13％）和6例（12％）中观察到TOP2alpha，HER2和p53基因的丢失。通过单因素分析，TOP2alpha指数（p = 0.0267），HER2评分（p = 0.028）和p53指数（p = 0.0188）显着，并且TOP2alpha基因的丢失（p = 0.0575）与肿瘤复发趋势相关，而HER2基因的丢失（p = 0.069）和p53基因的缺失（p = 0.0587）与肿瘤的发展趋势相关。在一个多变量分析中，其中还包括肿瘤分级和T1子期，TOP2alpha指数（p = 0.043）和p53指数（p = 0.02）被确定为肿瘤复发和p53基因缺失（p = 0.012）和T1子期的独立预测因子。 （p = 0.029）作为肿瘤进展的独立预测因子。结论：免疫组织化学TOP2alpha和p53染色以及p53基因拷贝数和T1亚型的FISH分析是提供有关T1过渡性细胞癌生物学行为的额外信息的有用手段。

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