...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Role of p21WAF1 in green tea polyphenol-induced growth arrest and apoptosis of oral carcinoma cells.
【24h】

Role of p21WAF1 in green tea polyphenol-induced growth arrest and apoptosis of oral carcinoma cells.

机译：p21WAF1在绿茶多酚诱导的口腔癌细胞生长停滞和凋亡中的作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cyclin-dependent kinase inhibitor p21WAF1 participates in cell growth, differentiation, and apoptosis. p21WAF1 can be induced by green tea polyphenol EGCG in several cancer cell types, but its role in the oral cancer cell response to EGCG is not known. We found that EGCG upregulates p21WAF1 in an oral carcinoma cell line, OSC2, by cDNA microarray. The current study determined the impact of siRNA-suppressed p21WAF1 and its response to EGCG on cell growth, DNA synthesis and apoptosis by RT-PCR, Western blot, BrdU incorporation, MTT and caspase 3 activity assays. Suppression of p21WAF1 by siRNA resulted in an accelerated cell growth and DNA synthesis, and increased cell viability. However, caspase 3 activity was not significantly inhibited. The evidence showed that p21WAF1 is involved in EGCG-induced growth arrest of OSC2 cells, which may facilitate caspase 3-mediated apoptosis. Thus, expression of functional p21WAF1 may promote phytochemical-mediated growth arrest and apoptosis in oral carcinoma cells.
机译：细胞周期蛋白依赖性激酶抑制剂p21WAF1参与细胞生长，分化和凋亡。绿茶多酚EGCG可以在几种癌细胞类型中诱导p21WAF1，但尚不清楚其在口腔癌细胞对EGCG反应中的作用。我们发现，EGCG通过cDNA微阵列上调了口腔癌细胞系OSC2中的p21WAF1。目前的研究通过RT-PCR，Western印迹，BrdU掺入，MTT和caspase 3活性测定法确定了siRNA抑制的p21WAF1及其对EGCG的反应对细胞生长，DNA合成和凋亡的影响。 siRNA抑制p21WAF1可以加速细胞生长和DNA合成，并提高细胞活力。但是，caspase 3活性没有被明显抑制。证据表明，p21WAF1与EGCG诱导的OSC2细胞生长停滞有关，这可能促进caspase 3介导的细胞凋亡。因此，功能性p21WAF1的表达可能促进植物化学介导的口腔癌细胞生长停滞和凋亡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号