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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Apoptosis of SAS cells induced by sonodynamic therapy using 5-aminolevulinic acid sonosensitizer.
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Apoptosis of SAS cells induced by sonodynamic therapy using 5-aminolevulinic acid sonosensitizer.

机译：使用5-氨基乙酰丙酸声敏剂的声波动力疗法诱导的SAS细胞凋亡。

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BACKGROUND: 5-Aminolevulinic acid (ALA) has been used as a photodynamic sensitizer for cancer treatment using photodynamic therapy. However, the light has markedly limited penetration depth. It was found that ALA also responds to low energy ultrasound, which has the capability to penetrate deep into tissues. Therefore, sonodynamic therapy (SDT) is a promising method for noninvasive treatment of tumors embedded deep in the tissue. It is desirable to kill the cancer cells via apoptosis rather than necrosis, and therefore, it is necessary to gain a better understanding of the mechanisms of treating cancer using SDT. MATERIALS AND METHODS: The apoptosis of SAS cells induced by pulsed 1.05MHz ultrasound in combination with ALA was investigated in vitro. RESULTS: The cells exposed to SDT with 10 mug/ml ALA displayed significantly higher apoptosis than cells treated by ultrasound alone. There was notably increased reactive oxygen species (ROS) production in the cells treated by SDT with ALA than by ultrasound alone, resulting in higher lipid peroxidation (LPO) level and more cells losing their mitochondrial membrane potential (MMP). CONCLUSION: ALA-mediated SDT produced strong apoptotic effects on SAS cells, which were mainly related to the excessive intracellular ROS production followed by LPO increase and MMP decrease.
机译：背景：5-氨基乙酰丙酸（ALA）已被用作使用光动力疗法治疗癌症的光动力敏化剂。然而，光的穿透深度明显受限。发现ALA还对低能量超声有反应，该超声具有穿透组织深层的能力。因此，声动力疗法（SDT）是一种有前途的方法，可用于无创性治疗组织深处埋藏的肿瘤。希望通过凋亡而不是坏死杀死癌细胞，因此，有必要更好地理解使用SDT治疗癌症的机制。材料与方法：体外研究了脉冲1.05MHz超声联合ALA诱导的SAS细胞凋亡。结果：与单独超声处理的细胞相比，暴露于SDT的10杯/毫升ALA的细胞显示出明显更高的凋亡。与单独使用超声相比，用ALA进行SDT处理的细胞中活性氧（ROS）的产生显着增加，从而导致更高的脂质过氧化（LPO）水平和更多的细胞丧失线粒体膜电位（MMP）。结论：ALA介导的SDT对SAS细胞产生强烈的凋亡作用，这主要与细胞内ROS产生过量，LPO升高和MMP降低有关。

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