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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells.
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Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells.

机译：氟硅酸钠处理在人成骨肉瘤（HOS）细胞中诱导凋亡。

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摘要

Fluorine compounds are widely used for the prevention of caries, and recently sodium fluorosilicate has been used in water fluorination. The cytotoxic effects of sodium fluorosilicate in several osteosarcoma and oral cancer cells were evaluated in this study by measurement of inhibition of cell proliferation. Human osteogenic sarcoma (HOS) cells were the most sensitive to sodium fluorosilicate treatment. Induction of apoptosis, such as nucleosomal DNA fragmentation and the appearance of apoptotic bodies, were observed in HOS cells by agarose gel electrophoresis and by flow cytometric analysis, respectively. The molecular mechanism of apoptosis induction in HOS was investigated by Western blot analysis. The level of Bcl-2 was decreased and consequent release of cytochrome c was increased. Caspase-3 was activated and the cleavage of poly (ADP-ribosyl) polymerase was increased. In conclusion, sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to thecytosol and activation of caspase-3.
机译：氟化合物广泛用于预防龋齿，近来氟硅酸钠已用于水氟化。在这项研究中，通过测量对细胞增殖的抑制作用，评估了氟硅酸钠对几种骨肉瘤和口腔癌细胞的细胞毒性作用。人成骨肉瘤（HOS）细胞对氟硅酸钠治疗最为敏感。通过琼脂糖凝胶电泳和流式细胞术分别在HOS细胞中观察到凋亡的诱导，例如核小体DNA片段化和凋亡小体的出现。通过Western blot分析了HOS诱导细胞凋亡的分子机制。 Bcl-2的水平降低，因此细胞色素c的释放增加。 Caspase-3被激活，聚（ADP-核糖基）聚合酶的切割增加。总之，氟硅酸钠通过Bcl-2的减少，细胞色素c向细胞质的释放以及caspase-3的活化来诱导HOS细胞凋亡。

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