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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene.
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Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene.

机译：在获取β-内酰胺酶bla KPC基因时的遗传结构。

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摘要

Genetic structures surrounding the carbapenem-hydrolyzing Ambler class A bla KPC gene were characterized in several KPC-positive Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from the United States, Colombia, and Greece. The bla KPC genes were associated in all cases with transposon-related structures. In the K. pneumoniae YC isolate from the United States, the beta-lactamase bla KPC-2 gene was located on a novel Tn3-based transposon, Tn4401. Tn4401 was 10 kb in size, was delimited by two 39-bp imperfect inverted repeat sequences, and harbored, in addition to the beta-lactamase bla KPC-2 gene, a transposase gene, a resolvase gene, and two novel insertion sequences, ISKpn6 and ISKpn7. Tn4401 has been identified in all isolates. However, two isoforms of this transposon were found: Tn4401a was found in K. pneumoniae YC and K. pneumoniae GR from the United States and Greece, respectively, and differed by a 100-bp deletion, located just upstream of the bla KPC-2 gene, compared to the sequence of Tn4401b, which was found in the Colombian isolates. In all isolates tested, Tn4401 was flanked by a 5-bp target site duplication, the signature of a recent transposition event, and was inserted in different open reading frames located on plasmids that varied in size and nature. Tn4401 is likely at the origin of carbapenem-hydrolyzing beta-lactamase KPC mobilization to plasmids and its further insertion into various-sized plasmids identified in nonclonally related K. pneumoniae and P. aeruginosa isolates.
机译：在从美国，哥伦比亚和希腊分离的几种KPC阳性肺炎克雷伯菌和铜绿假单胞菌菌株中鉴定了碳青霉烯水解Ambler A类bla KPC基因周围的遗传结构。 bla KPC基因在所有情况下都与转座子相关结构相关。在来自美国的肺炎克雷伯菌YC分离株中，β-内酰胺酶bla KPC-2基因位于新型的基于Tn3的转座子Tn4401上。 Tn4401的大小为10 kb，由两个39 bp的不完美反向重复序列界定，除了β-内酰胺酶bla KPC-2基因外，还包含转座酶基因，resolvase基因和两个新的插入序列ISKpn6和ISKpn7。 Tn4401已在所有分离株中鉴定。但是，发现了该转座子的两个同工型：Tn4401a在分别来自美国和希腊的肺炎克雷伯氏菌YC和肺炎克雷伯氏菌GR中发现，并且有一个100 bp的缺失，位于bla KPC-2的上游该基因与哥伦比亚分离物中发现的Tn4401b序列相比。在所有测试的分离物中，Tn4401侧翼有一个5 bp的目标位点重复，这是最近发生的转座事件的特征，并被插入到大小和性质不同的质粒上的不同开放阅读框中。 Tn4401可能是将碳青霉烯水解β-内酰胺酶KPC动员到质粒的起点，并将其进一步插入非克隆相关肺炎克雷伯菌和铜绿假单胞菌分离株中鉴定的各种大小的质粒中。

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