Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

Cross NA; Kellock DJ; Kinghorn GR; Taraktchoglou M; Bataki E; Oxley KM; Hawkey PM; Eley A

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Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

机译：沙眼衣原体的抗菌药敏试验采用基于逆转录PCR的方法。

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The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).

机译：沙眼衣原体抗菌药敏试验的常规方法是主观的，并且可能会引起误解。我们已经开发了一种基于逆转录酶PCR（RT-PCR）的方法，该方法比传统方法灵敏且主观性较低。在三份测定中使用16株沙眼衣原体菌株，我们发现RT-PCR方法对所有八种抗菌药物的敏感性始终比传统技术更高，通过RT-PCR测定的结果MIC从高1.6倍（红霉素）到> / =高195倍（阿莫西林）。

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《Antimicrobial agents and chemotherapy.》 |1999年第9期|共3页
作者
Cross NA; Kellock DJ; Kinghorn GR; Taraktchoglou M; Bataki E; Oxley KM; Hawkey PM; Eley A;
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正文语种 eng
中图分类治疗学;
关键词
Amoxicillin; Antibiotics; Macrolide; Chlamydia trachomatis; Erythromycin; 阿莫西林; 抗生素; 大环内酯; 衣原体; 沙眼; 红霉素; 青霉素类; 逆转录聚合酶链反应;

机译：Amoxicillin;Antibiotics;Macrolide;Chlamydia trachomatis;Erythromycin;阿莫西林;抗生素;大环内酯;衣原体;沙眼;红霉素;青霉素类;逆转录聚合酶链反应;

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1. Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method. [J] . Cross NA, Kellock DJ, Kinghorn GR, Antimicrobial agents and chemotherapy. . 1999,第9期

机译：沙眼衣原体的抗菌药敏试验采用基于逆转录PCR的方法。
2. Phenotypic antimicrobial susceptibility testing of Chlamydia trachomatis isolates from patients with persistent or successfully treated infections [J] . RachelPitt, SarahAlexander, CatherineIson, The Journal of Antimicrobial Chemotherapy . 2018,第3期

机译：患有持续或成功治疗感染患者的衣原体抗菌剂的表型抗菌易患性试验
3. Improved Syva MicroTrak Chlamydia trachomatis direct test method. [J] . B A Judson, P P Lambert Journal of Clinical Microbiology . 1988,第12期

机译：改良的Syva MicroTrak沙眼衣原体直接检测方法。
4. Testing for adventitious retroviruses using PCR-based reverse transcriptase assays [C] . LOVATT A., BLACK J., GALBRAITH D. Annual Meeting of the European Society of Animal Cell Technology . 1999

机译：使用基于PCR的逆转录酶测定来测试外来逆转录病毒
5. In vitro susceptibility of Chlamydia trachomatis to LPS-binding polyamines and cellulose ether polymers: towards the development of a microbicide against Chlamydia infection. [D] . Osaka, Ichie. 2013

机译：沙眼衣原体对LPS结合多胺和纤维素醚聚合物的体外敏感性：趋向于开发一种抗衣原体感染的杀菌剂。
6. Note: Antimicrobial Susceptibility Testing of Chlamydia trachomatis Using a Reverse Transcriptase PCR-Based Method [O] . N. A. Cross, D. J. Kellock, G. R. Kinghorn, 1999

机译：注意：使用基于逆转录酶PCR的方法对沙眼衣原体进行药敏试验
7. A reverse transcriptase-PCR based assay for in-vitro antibiotic susceptibility testing of Chlamydia pneumoniae [O] . M. A. Khan, C. W. Potter, R. M. Sharrard 1996

机译：基于氯酰胺肺炎的体外抗生素敏感性试验的逆转录酶-PCR基于逆转录酶-PCR

Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

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