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首页> 外文期刊>Journal of cellular physiology. >Zinc supplementation alleviates endoplasmic reticulum stress during porcine oocyte in vitro maturation by upregulating zinc transporters
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Zinc supplementation alleviates endoplasmic reticulum stress during porcine oocyte in vitro maturation by upregulating zinc transporters

机译：补锌减轻内质的网在猪卵母细胞体外应力成熟的移植锌转运蛋白

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摘要

Endoplasmic reticulum (ER) stress is a major contributor to embryonic development failure. Mammalian oocytes have a high risk of exposure to cellular stress during in vitro embryo production. We investigated the effects of zinc supplementation during in vitro maturation under ER stress. We evaluated cumulus expansion, embryonic development derived by parthenogenetic activation, reactive oxygen species, protein expression of X-box binding protein 1 (XBP1), and expression of genes related to ER stress. Supplementation with 1μg/ml zinc significantly increased the nuclear maturation of oocytes, cleavage and blastocyst formation rates, and total blastocyst cell number (p < .05). Under ER stress, zinc significantly reduced protein expression of XBP1, and increased cleavage and blastocyst rates (p < .05). Concomitantly, zinc supplementation upregulated the expression of zinc transporters (SLC39A14 and SLC39A10), PTGS2, and downregulated ER stress-related genes (sXBP1, uXBP1, ATF4, and PTPN1/PTP1B), and caspase 3. These results suggest that zinc supplementation alleviates ER stress by providing essential metal-ion transporters for oocyte maturation and subsequent embryonic development.
机译：压力是一个主要的内质网(ER)贡献者胚胎发育失败。哺乳动物卵母细胞有很高的投资风险在体外胚胎细胞压力生产。补充在体外成熟ER应激。通过孤雌生殖的胚胎发育中激活、活性氧、蛋白质表达式x - box的结合蛋白1 (XBP1)ER应激相关的基因的表达。补充1μg / ml锌显著增加了卵母细胞的核成熟,乳沟和囊胚形成率总囊胚细胞数(p < . 05)。压力,锌显著降低蛋白质XBP1的表达式,乳沟和增加囊胚率(p < . 05)。补充调节的表达和表达下调ER应激相关基因(sXBP1uXBP1、ATF4 PTPN1 /应用PTP1B)和半胱天冬酶3。这些结果表明,补锌通过提供基本缓解ER应激对卵母细胞成熟和金属离子转运蛋白后续的胚胎发育。

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