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首页> 外文期刊>Journal of cellular physiology. >Melatonin protects against defects induced by malathion during porcine oocyte maturation
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Melatonin protects against defects induced by malathion during porcine oocyte maturation

机译：褪黑素可防止缺陷引起的马拉松在猪卵母细胞成熟

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摘要

Malathion (MAL) is a common organophosphorus pesticide and affects both animal and human reproduction. However, the mechanisms regarding how MAL affects the mammalian oocyte quality and how to prevent it have not been fully investigated. In this study, we used porcine oocyte as a model and proved that MAL impaired porcine oocyte quality in a dose-dependent manner during maturation. MAL decreased the first polar body extrusion, disrupted spindle assembly and chromosome alignment, impaired cortical granules (CGs) distribution, and increased reactive oxygen species (ROS) level in oocytes. RNA-seq analysis showed that MAL exposure altered the expression of 2,917 genes in the porcine maturated oocytes and most genes were related to ROS, the lipid droplet process, and the energy supplement. Nevertheless, these defects could be remarkably ameliorated by adding melatonin (MLT) into the oocyte maturation medium. MLT increased oocyte maturation rate and decreased the abnormities of spindle assembly, CGs distribution and ROS accumulation in MAL-exposed porcine oocytes. More important, MLT upregulated the expression of genes related to lipid droplet metabolism (PPARγ and PLIN2), decreased lipid droplet size and lipid peroxidation in MAL-exposed porcine oocytes. Finally, we found that MLT increased the blastocysts formation and the cell numbers of blastocysts in MAL-exposed porcine oocytes after parthenogenetic activation, which was mediated by reduction of ROS levels and maintaining lipid droplet metabolism. Taken together, our results revealed that MLT had a protective action against MAL-induced deterioration of porcine oocyte quality.
机译：马拉松(MAL)是一种常见的有机磷农药和影响动物和人类繁殖。哺乳动物卵母细胞质量和MAL如何影响如何防止它尚未完全调查。卵母细胞作为模型,并证明了MAL受损猪卵母细胞质量的剂量依赖性的方式在成熟。身体的挤压,纺锤体组装和中断染色体定位、皮质颗粒受损(CGs)分布,增加了活性氧物种的卵母细胞(ROS)水平。表明,MAL暴露改变了表情2917个基因的猪成熟卵母细胞和大多数基因与ROS,脂质液滴的过程,能量补充。然而,这些缺陷可能是显著的通过添加褪黑素(MLT)到改善卵母细胞成熟的媒介。成熟率和降低的异常纺锤体组装,CGs分布和活性氧积累在MAL-exposed猪卵母细胞。重要的是,MLT调节的表达与脂滴代谢相关基因(PPARγ和PLIN2),脂滴的大小和下降在MAL-exposed猪脂质过氧化作用卵母细胞。胚泡形成和细胞的数量胚泡在MAL-exposed猪卵母细胞孤雌生殖的激活,这是由减少ROS水平和维护脂质液滴的新陈代谢。透露,MLT保护行动MAL-induced猪卵母细胞的恶化质量。

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