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首页> 外文期刊>Journal of cellular physiology. >miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN
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miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN

机译：mir - 298在结肠癌中起着举足轻重的作用侵袭性通过瞄准PTEN

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Evidence indicate that the miR-298 dysregulation might associate with colorectal cancer (CRC) development. Herein, we evaluated the effect of miR-298 dysregulation on colon cancer invasiveness. First, metabolic activity, cell cycle progression, apoptosis, and invasion of miR-298 overexpressed/knocked out colon cancer cells were examined and combined with their transcriptome analysis data for better visualization of miR-298 intracellular signaling networks. Interaction between miR-298 and its target was evaluated with luciferase assay and validated using western blot analysis. The proportion of abnormal miR-298 level was investigated in tumor samples, matched normal adjacent tissues, and plasmas of 100 CRC patients, and also compared with 100 normal plasma samples. The Mann-Whitney U test was performed to assess miR-298 differences among the studied groups, and the correlation between miR-298 and the risk of CRC was shown by univariate and multivariate logistic regression. The data indicate that miR-298 overexpression promoted proliferation and metastasis in CRC cells via targeting phosphatase and tensin homolog. Comparative analysis of CRC tumors, normal adjacent tissues, and plasmas indicated a significant miR-298 upregulation in tumors and plasmas (1.72-fold and 1.65-fold, respectively; p<.001). Also, the aberrant level of miR-298 contributed with CRC tumor differentiation, TNM stage and lymph node metastasis (p < .001), and independently associated with poor survival of CRC patients (p<.029; hazard ratio: 1.292; 95% confidence interval: 0.339-2.184). Collectively, these data showed that abnormal level of miR-298 correlated with cancer development and through that lowered the overall survival rate of CRC patients. Therefore, miR-298 could be considered as a therapeutic target for CRC.
机译：证据表明,mir - 298失调可能与结直肠癌(CRC)发展。在结肠癌mir - 298失调侵袭性。循环过程、细胞凋亡和入侵mir - 298中/淘汰结肠癌细胞被检查和结合为更好的转录组分析数据mir - 298细胞内信号的可视化网络。目标与荧光素酶试验和评估利用免疫印迹分析进行验证。的比例异常mir - 298水平研究肿瘤样本,与正常邻近组织,100 CRC的等离子体与100年相比病人,也正常血浆样品。执行评估mir - 298之间的差异研究团体和之间的关系mir - 298和显示了CRC的风险单变量和多变量逻辑回归。数据表明,mir - 298超表达促进了CRC的扩散和转移通过针对磷酸酶和tensin细胞同族体。邻近正常组织和等离子体显示在肿瘤和重大mir - 298 upregulation等离子体(分别为1.72倍和1.65倍;p <措施)。贡献与CRC肿瘤分化,TNM舞台和淋巴结转移(p <措施)独立与贫穷的生存有关CRC患者(p < .029;置信区间:0.339—-2.184)。这些数据表明,mir - 298水平异常与癌症发展这降低了CRC的总体生存率病人。作为CRC的治疗目标。

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