...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >A Stable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of the Medicinal Plant Digitalis purpurea L.
【24h】

A Stable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of the Medicinal Plant Digitalis purpurea L.

机译：一种稳定高效的根癌农杆菌介导的药用植物洋地黄紫的遗传转化。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we developed a rapid and efficient method for in vitro propagation and Agrobacterium tumefaciens-mediated transformation of Digitalis purpurea L. (syn. foxglove), an important medicinal plant. Mature leaf explants of D. purpurea were used for 100 % adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 1 mg L~(?1) thidiazuron (TDZ) (a cytokine) and 0.1 mg L~(?1) 1-naphthaleneacetic acid (NAA) (an auxin). Transformation was achieved by inoculating leaf explants with the A. tumefaciens strains GV2260/pBI121 or GV3101/pBI121. The binary vector pBI121 contained the reporter β-glucuronidase gene (GUS) and kanamycin selection marker nptII. Kanamycin-resistant shoots were regenerated directly on the selection medium 4–6 weeks after co-cultivation. Approximately, 52.2 and 60 % of kanamycin-resistant shoots transformed with Agrobacterium strains GV2260 and GV3101, respectively, showed strong GUS staining by histochemical assay. Furthermore, PCR and Southern blot analysis confirmed the presence of nptII and GUS on the chromosome of the transformed D. purpurea plants, and stable GUS expression was detected in the transformants by RT-PCR analysis. This efficient method of shoot regeneration and genetic transformation of D. purpurea will provide a powerful tool to increase and produce valuable components such as digitoxin, digoxin, and digoxigenin in D. purpurea through improved secondary metabolic pathways via a biotechnological approach.
机译：在这项研究中，我们开发了一种快速有效的方法，用于体外繁殖和根癌农杆菌介导的重要药用植物洋地黄（Syn。foxglove）的转化。在添加了1 mg L〜（？1）噻二唑隆（TDZ）（一种细胞因子）和0.1 mg L〜（？1）的Murashige和Skoog（MS）培养基上，紫叶菊的成熟叶片外植体用于100％不定芽再生。 1-萘乙酸（NAA）（一种生长素）。通过用根癌农杆菌菌株GV2260 / pBI121或GV3101 / pBI121接种叶外植体来实现转化。二元载体pBI121包含报告基因β-葡萄糖醛酸酶基因（GUS）和卡那霉素选择标记nptII。共培养4-6周后，耐卡那霉素的芽直接在选择培养基上再生。通过组织化学测定，分别用农杆菌菌株GV2260和GV3101转化的卡那霉素抗性芽的分别约52.2和60％显示出强的GUS染色。此外，PCR和Southern印迹分析证实了在转化的紫癜植物的染色体上存在nptII和GUS，并且通过RT-PCR分析在转化体中检测到稳定的GUS表达。这种有效的紫茎泽兰再生和遗传转化方法将通过生物技术途径改善次级代谢途径，为紫茎泽兰增加和生产有价值的成分，如洋地黄毒苷，地高辛和洋地黄毒苷，提供了有力工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号