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首页> 外文期刊>Archiv der Pharmazie >Adaptation of DELFIA cortisol kit for determination of salivary cortisol concentration.
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Adaptation of DELFIA cortisol kit for determination of salivary cortisol concentration.

机译：调整DELFIA皮质醇试剂盒以确定唾液皮质醇浓度。

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Salivary cortisol concentration seems to be an excellent indicator of the biologically active plasma cortisol level because of the nearly absence of corticoid-binding proteins in saliva. With regard to the easy, noninvasive, and stress-free nature of saliva sampling, this parameter greatly facilitates studies of the HPA (hypothalamus-pituitary-adrenocortical) axis, especially in children. A commercially available TR-FIA (time-resolved fluoroimmunoassay), the DELFIA (dissociation-enhanced lanthanide fluoroimmunoassay) method, proposed for plasma and urine cortisol analyses, was adapted for salivary cortisol measurement by means of simple modifications of the assay protocol. The sensitivity was determined to be 0.53 nmol/L. The intra- and interassay coefficients of variation ranged from 5.5 to 8.2 % and from 6.0 to 10.4 %, respectively. The present findings suggest that the DELFIA procedure provides a reliable, sensitive, and convenient alternative procedure to the assay for salivary cortisol.
机译：唾液皮质醇浓度似乎是生物活性血浆皮质醇水平的极佳指标，因为唾液中几乎不存在皮质类固醇结合蛋白。关于唾液采样的简便，无创和无压力的性质，该参数极大地促进了HPA（下丘脑-垂体-肾上腺皮质激素）轴的研究，特别是在儿童中。提议对血浆和尿液中的皮质醇进行分析的市售TR-FIA（时间分辨荧光免疫分析），DELFIA（解离增强镧系元素荧光免疫分析）方法适用于唾液皮质醇的测量，方法是对试验方案进行了简单的修改。灵敏度确定为0.53 nmol / L。批内和批间变异系数分别为5.5％至8.2％和6.0％至10.4％。目前的发现表明，DELFIA程序为唾液皮质醇的测定提供了可靠，灵敏和方便的替代程序。

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