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首页> 外文期刊>Aquatic Biology >Heat stress-mediated gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus
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Heat stress-mediated gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus

机译：日本海参Apostichopus japonicus体壁中热应激介导的基因表达

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摘要

Suppression subtractive hybridization was used to analyze the differences in gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus, induced by heat stress (28掳C), in order to understand the response at the molecular level. A total of 737 clones was selected and analyzed, and 165 distinct sequences were obtained, with 65 sharing similarity to sequences in public databases. The sequenced genes were classified into 3 functional categories. Increased expression of some genes in response to stress was concomitant with decreased expression of genes concerned with energy production and metabolism. The results (1) suggest that heat stress has significant effects on gene expression in A. japonicus, and (2) provide the basis for further study of molecular regulation mechanisms underlying the response to heat stress and estivation in A. japonicus.
机译：为了研究在分子水平上的响应，使用抑制消减杂交技术分析了日本海参（Apostichopus japonicus）在热应激（28掳C）诱导下体壁基因表达的差异。共选择和分析了737个克隆，获得了165个不同的序列，其中65个与公共数据库中的序列具有相似性。测序的基因分为3个功能类别。一些基因响应压力的表达增加与能量生产和代谢有关的基因表达减少。结果（1）表明热应激对日本刺槐的基因表达有显着影响，（2）为进一步研究分子结构调控机制奠定了基础，该分子调控机制是对日本刺猬对热应激和诱变的反应所依据的。

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