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首页> 外文期刊>BMB Reports >Beta-carboline alkaloids harmaline and harmalol induce melanogenesis through p38 mitogen-activated protein kinase in B16F10 mouse melanoma cells
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Beta-carboline alkaloids harmaline and harmalol induce melanogenesis through p38 mitogen-activated protein kinase in B16F10 mouse melanoma cells

机译：β-咔啉生物碱harmaline和harmalol通过p38丝裂原激活的蛋白激酶在B16F10小鼠黑色素瘤细胞中诱导黑色素生成

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摘要

Melanin synthesis is regulated by melanocyte specific enzymes and related transcription factors. beta-carboline alkaloids including harmaline and harmalol are widely distributed in the environment including several plant families and alcoholic beverages. Presently, melanin content and tyrosinase activity were increased in melanoma cells by harmaline and harmalol in concentration- and time-dependent manners. Increased protein levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were also evident In addition, immunofluorescence and Western blot analyses revealed harmaline and harmalol increased cAMP response element binding protein phosphorylation and microphthalmia-associated transcription factor expression. In addition to studying the signaling that leads to melanogenesis, roles of the p38 MAPK pathways by the harmaline and harmalol were investigated. Harmaline and harmalol induced time-dependent phosphorylation of p38 MAPK. Harmaline and harmalol stimulated melanin synthesis and tyrosinase activity, as well as expression of tyrosinase and TRP-1 and TRP-2 indicating that these harmaline and harmalol induce melanogenesis through p38 MAPK signaling.
机译：黑色素的合成受黑色素细胞特异性酶和相关转录因子的调节。 β-咔啉生物碱（包括harmaline和harmalol）广泛分布于环境中，包括几种植物科和含酒精的饮料。目前，harmaline和harmalol以浓度和时间依赖性方式增加了黑色素瘤细胞中黑色素的含量和酪氨酸酶活性。酪氨酸酶，酪氨酸酶相关蛋白-1（TRP-1）和TRP-2的蛋白水平也明显升高。此外，免疫荧光和Western印迹分析显示，harmaline和harmalol增加了cAMP反应元件结合蛋白的磷酸化和与小眼症相关的转录因子表达。除了研究导致黑色素生成的信号传导外，还研究了harmaline和harmalol对p38 MAPK途径的作用。 Harmaline和harmalol诱导p38 MAPK的时间依赖性磷酸化。 Harmaline和harmalol刺激黑色素的合成和酪氨酸酶活性，以及​​酪氨酸酶和TRP-1和TRP-2的表达，表明这些harmaline和harmalol通过p38 MAPK信号传导诱导黑色素生成。

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