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首页> 外文期刊>Aquaculture >Ontogenetic development of the digestive tract in reared spotted sand bass Paralabrax maculatofasciatus larvae
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Ontogenetic development of the digestive tract in reared spotted sand bass Paralabrax maculatofasciatus larvae

机译：养斑斑鲈Paralabrax maculatofasciatus幼虫消化道的发育发育

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Histological and histochemical methods were used to describe the development of the digestive tract in spotted sand bass larvae reared under culture conditions. The larvae were kept in six 100-l tanks for 30 days. They were fed with Brachionus plicatilis from day 2 after hatching to day 13; Artemia nauplli from day 12 to day 17; Artemia juveniles and adults from day 14 to day 26; and a semi-dry artificial diet from day 20 onwards. Development of the digestive tract in spotted sand bass followed the general pattern described for other species. At hatching, it consisted of an undifferentiated straight tube laying over the yolk sac. At first feeding (day 2), the yolk sac was partially depleted and the mouth and anus were open. On day 5, the digestive tract was fully differentiated into buccopharynx, esophagus, stomach, anterior intestine, posterior intestine, and rectum. A few days after the onset of first feeding, a PAS-positive reaction was observed in both intestinal mucosae. The liver showed a constant glycogen accumulation after day 4. Formation of several supranuclear ninhydrin-Schiff (NS) positive vacuoles was observed in the enterocytes of the posterior intestine. Large supranuclear vacuoles were present in the enterocytes of the anterior intestine. The gastric glands and pyloric caeca appeared on day 16. Vacuoles of both intestines decreased in size and number with the appearance of gastric glands. A probable modification of the actual feeding schedule of spotted sand bass larvae is proposed.
机译：组织学和组织化学方法用来描述在养殖条件下饲养的斑点鲈鱼幼虫消化道的发育。将幼虫在六个100升的水箱中放置30天。从孵化后的第2天到第13天，向他们喂食褶Bra。第12天至第17天有无节制性贫血;第14天至第26天有卤虫少年和成年人;以及从第20天开始的半干人工饮食。斑点鲈鱼中消化道的发育遵循其他物种的一般模式。孵化时，它由铺在卵黄囊上的未分化直管组成。初次喂养（第2天）时，卵黄囊部分耗尽，嘴和肛门张开。在第5天，消化道完全分化为食道，食道，胃，前肠，后肠和直肠。首次进食开始几天后，在两个肠粘膜中均观察到PAS阳性反应。在第4天后，肝脏显示出恒定的糖原积累。在后肠的肠上皮细胞中观察到几个核上茚三酮-希夫（NS）阳性液泡的形成。大肠核液泡存在于前肠的肠细胞中。在第16天出现胃腺和幽门盲肠。随着肠胃的出现，两个肠的小泡大小和数量均减少。提出了可能对斑spot鲈幼虫实际进食时间表的修改。

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