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首页> 外文期刊>Journal of Cellular Physiology >Spermicidal efficacy of VRP, a synthetic cationic antimicrobial peptide, inducing apoptosis and membrane disruption
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Spermicidal efficacy of VRP, a synthetic cationic antimicrobial peptide, inducing apoptosis and membrane disruption

机译：合成阳离子VRP的杀精子的功效抗菌肽,诱导细胞凋亡膜的破坏

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摘要

Presently available contraceptives are mostly hormonal or detergent in nature with numerous side effects like irritation, lesion, inflammation in vagina, alteration of body homeostasis, etc. Antimicrobial peptides with spermicidal activity but without adverse effects may be suitable alternatives. In the present study, spermicidal activity of a cationic antimicrobial peptide VRP on human spermatozoa has been elucidated. Progressive forward motility of human spermatozoa was instantly stopped after 100?μM VRP treatment and at 350?μM, all kinds of sperm motility ceased within 20?s as assessed by the Sander–Cramer assay. The spermicidal effect was confirmed by eosin–nigrosin assay and HOS test. VRP treatment (100?μM) in human spermatozoa induced both the intrinsic and extrinsic pathways of apoptosis. TUNEL assay showed VRP treatment significantly disrupted the DNA integrity and changed the mitochondrial membrane permeability as evident from MPTP assay. AFM and SEM results depicted ultra structural changes including disruption of the acrosomal cap and plasma membrane of the head and midpiece region after treatment with 350?μM VRP. MTT assay showed after treatments with 100 and 350?μM of VRP for 24?hr, a substantial amount of Lactobacillus acidophilus (about 90% and 75%, respectively) remained viable. Hence, VRP being a small synthetic peptide with antimicrobial and spermicidal activity but tolerable to normal vaginal microflora, may be a suitable target for elucidating its contraceptive potentiality.
机译：避孕药主要是目前可用在自然界中许多激素或的洗衣粉副作用喜欢刺激、损伤阴道炎症,身体的改变体内平衡等。杀精子的活动但是没有不利影响可能是合适的选择。研究中,阳离子的杀精子的活动抗菌肽VRP人类精子已被阐明。人类的精子后立即停止100年?精子的运动性停止在20吗?Sander-Cramer化验。证实了eosin-nigrosin化验和居屋计划吗测试。诱导的内在和外在的途径细胞凋亡。显著破坏DNA完整性和改变了线粒体膜透性注射明显从MPTP药物试验。超结构变化包括中断acrosomal帽和等离子体膜后的头和中段地区治疗350 ?与100年和350年的治疗方法?大量的嗜酸乳杆菌(分别为90%和75%)可行的。肽抗菌和杀精子的但正常阴道活动还可以忍受微生物区系,可能是一个合适的目标阐明其避孕潜力。

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