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首页> 外文期刊>Journal of Cellular Physiology >Gingival spheroids possess multilineage differentiation potential
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Gingival spheroids possess multilineage differentiation potential

机译：牙龈拥有multilineage球状体分化潜能

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Recently studies have demonstrated HGMSCs as ideal candidates for regenerative study. Interestingly we found that HGMSCs derived spheroids are more potent and maintain the properties of stemness convincingly compared to conventional culture methods. During the culture, GMSCs instinctively accumulated into spheroids and display multipotent STRO‐1 and Vimentin‐positive cells. Reduced phenotypic expression of CD73, CD105, and elevated expression STRO‐1 and CD‐34. Pluripotent nature of S‐GMSCs putatively shown the expression of OCT4A, NANOG, SOX‐2, SSEA4, TRA‐1‐60, and TRA‐181. Also, levels of protein are much higher in spheroid than dissociated culture. On endothelial induction, spheroid differentiated and developed a vascular structure with positive expression of CD31 and on neuronal induction showed positivity for TUJ1 and E‐Cadherin. Importantly, undifferentiated state of S‐GMSCs exhibited significant upregulation of aforementioned pluripotent genes and lack of pro‐inflammatory cytokines IL‐6 and amplified ARF signal confirming that the spheroids are not teratoma formation. However, higher of CAP1, CP, TGFβ, OPN, PPAR?, TUJ1, and NESTIN expression observed in spheroids, and minimal expression of the same markers were observed in adherent GMSCs respectively. Ahead of dissociated gingival culture, spheroid provides enhanced viable, pluripotent, and multilineage ability. This study suggested that S‐GMSCs increased the chances of therapeutic efficacy in the regenerative applications.
机译：最近的研究已经证明HGMSCs理想候选人再生研究。我们发现HGMSCs衍生更球状体有效的和维护具备干细胞的特性令人信服地比传统文化方法。积累成球状体和显示多功能STRO 1和波形蛋白阳性细胞。减少表型的表达CD73、CD105和高表达STRO 1和CD 34。年代的本质特色业务推定地显示表达式OCT4A, NANOG的袜‐2、SSEA4 TRA‐1‐60,和交易181。在球体的文化。内皮感应,球体有区别和发展积极的血管结构CD31表达和神经感应显示TUJ1积极性和E钙粘素。重要的是,S的未分化状态的业务表现出显著upregulation提到的多能性基因和缺乏箴炎性细胞因子IL 6和放大ARF信号确认不是球状体畸胎瘤的形成。TGFβ,OPN, PPAR ?在球状体,最小的表达相同的标记观察附着业务分别。文化、球体提供了增强的可行的多能,multilineage能力。建议年代特色业务的可能性增加再生的治疗效果应用程序。

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