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首页> 外文期刊>Archives of dermatological research. >Glucocorticoids inhibit proliferation and adhesion of the IL-3-dependent mast cell line, MC/9, to NIH/3T3 fibroblasts, with an accompanying decrease in IL-3 receptor expression.
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Glucocorticoids inhibit proliferation and adhesion of the IL-3-dependent mast cell line, MC/9, to NIH/3T3 fibroblasts, with an accompanying decrease in IL-3 receptor expression.

机译:糖皮质激素抑制IL-3依赖性肥大细胞系MC / 9增殖和粘附至NIH / 3T3成纤维细胞,并伴随IL-3受体表达降低。

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摘要

We investigated the effects of glucocorticoids on IL-3-dependent proliferation and c-kit expression of cells of the mouse mast cell line, MC/9. Glucocorticoids (dexamethasone, prednisolone, and hydrocortisone) inhibited IL-3-dependent MC/9 cell proliferation, whereas sex steroids (progesterone, beta-estradiol, and testosterone) had no effect. Flow cytometric analysis revealed that glucocorticoids reduced the expression of the IL-3 receptor on MC/9 cells. Immunoblot and Northern blot analyses indicated that glucocorticoids also reduced the expression of both c-kit protein and c-kit mRNA transcript. Furthermore, the adhesion of MC/9 cells to stem cell factor-expressing NIH/3T3 cells was reduced following glucocorticoid treatment. Our results indicate that glucocorticoids inhibit IL-3-dependent MC/9 mast cell proliferation, with an accompanying decrease in IL-3 receptor expression. Glucocorticoids also reduced c-kit expression on MC/9 cells resulting in a decreased adhesion to NIH/3T3 fibroblasts.
机译:我们研究了糖皮质激素对小鼠肥大细胞系MC / 9的IL-3依赖性增殖和c-kit表达的影响。糖皮质激素(地塞米松,泼尼松龙和氢化可的松)抑制依赖IL-3的MC / 9细胞增殖,而性类固醇(孕酮,β-雌二醇和睾丸激素)无效。流式细胞仪分析表明,糖皮质激素降低了MC / 9细胞上IL-3受体的表达。免疫印迹和RNA印迹分析表明,糖皮质激素还降低了c-kit蛋白和c-kit mRNA转录物的表达。此外,糖皮质激素治疗后降低了MC / 9细胞与表达干细胞因子的NIH / 3T3细胞的粘附。我们的结果表明,糖皮质激素抑制IL-3依赖的MC / 9肥大细胞增殖,并伴随IL-3受体表达的降低。糖皮质激素还降低了MC / 9细胞上的c-kit表达,导致与NIH / 3T3成纤维细胞的粘附性降低。

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