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首页> 外文期刊>Archives des sciences >Assessing efficacy of ultra-filtration and slow filtration in soil-less production by molecular detection of Pythium oligandrum and Bacillus subtilis as model organisms
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Assessing efficacy of ultra-filtration and slow filtration in soil-less production by molecular detection of Pythium oligandrum and Bacillus subtilis as model organisms

机译：通过低聚腐霉菌和枯草芽孢杆菌作为模型生物的分子检测评估超滤和慢滤在无土生产中的功效

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Greenhouse crops are mainly grown on artificial substrates in soil-less systems. Re-circulating nutrient systems offer an advantageous alternative for controlling nutrient solutions leaching from greenhouses into the environment, which represents an environmental issue in greenhouse production areas. However, the potential for the rapid spread of plant pathogens, is the main hindrance to the general adoption of re-circulating nutrient systems by the greenhouse industry. Here we assessed by real time PCR the efficacy of filtration for eliminating oomycete and bacterial pathogens, artificially added in the re-circulating nutrient solution, by using a tangential ultra-filtration system, a slow sand filter (SSF) and a slow Rockwool granule filter (SRF). The oomycete Pythium oligandrum and the bacterium Bacillus subtilis were used as model organisms because of their easy culture, their lack of pathogenicity and their similarity with common oomycete and bacterial plant pathogens. Using real time PCR and species specific oligonucleotides showed that ultra-filtration and SRF filter were highly effective in removing P oligandrum and B. subtilis, while the SSF although completely removing F oligandrum, was less effective for B. subtilis removal. Real time PCR showed to be a much quicker alternative to classical counting on agar plate culture.
机译：温室作物主要生长在无土壤系统的人工基质上。再循环养分系统为控制从大棚浸出到环境中的养分溶液提供了一种有利的替代方法，这代表了温室生产区的环境问题。然而，植物病原体快速传播的潜力是温室产业普遍采用再循环养分系统的主要障碍。在这里，我们通过切向超滤系统，慢速砂滤器（SSF）和慢速Rockwool颗粒过滤器，通过实时PCR评估了过滤在消除循环菌营养液中人工添加的卵菌和细菌病原体方面的功效。 （SRF）。卵菌腐霉和枯草芽孢杆菌由于其易于培养，缺乏致病性以及与常见卵菌和细菌性植物病原体的相似性而被用作模型生物。使用实时PCR和物种特异性寡核苷酸显示，超滤和SRF过滤器在去除P oligandrum和枯草芽孢杆菌方面非常有效，而SSF尽管完全去除了F oligandrum，但对枯草芽孢杆菌的去除效果较差。实时PCR显示出比琼脂平板培养经典计数更快的替代方法。

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