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首页> 外文期刊>Acta Horticulturae >Assessing Efficacy of Ultra-Filtration and Bio-Filtration Systems Used in Soilless Production through Molecular Detection of Pythium oligandrum and Bacillus subtilis as Model Organisms
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Assessing Efficacy of Ultra-Filtration and Bio-Filtration Systems Used in Soilless Production through Molecular Detection of Pythium oligandrum and Bacillus subtilis as Model Organisms

机译：通过对低聚腐霉菌和枯草芽孢杆菌作为模型生物进行分子检测来评估无土生产中使用的超滤和生物过滤系统的功效

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The majority of greenhouse crops are grown on artificial substrates in hydroponic systems. Re-circulating nutrient systems offer an advantageous alternative for controlling nutrient solutions leaching from greenhouses into the environment, which represents an environmental issue in greenhouse production areas. However, the potential for the rapid spread of root diseases, and other diseases, is the main hindrance to the general adoption of re-circulating nutrient systems by the greenhouse industry. Inthis study, the total bacterial, fungal and oomycete communities present in re-circulating nutrient solutions, were monitored using selective media and direct viable counts. Sequence analysis of 126 main representatives of these three communities showedfor Eubacteria the presence of 11 genera of y-Proteobacteria, 3 genera of Firmicutes, one genus of Actinobacteria and one genus of Flavobacteria. For the fungi, recovered strains belonged to Rhizopus and to Postia. Twenty strains belonged to the Stramenopile lineage, 19 of them to the genus Pythium (Oomycota) and one to the genus Splachnidium (Phaeophyceae). In addition, the efficacy of ultra-filtration and bio-filtration for eliminating oomycete and bacterial pathogens, artificially added in the re-circulating nutrient solution was assessed, by using a tangential ultrafiltration system, a slow sand filter and a rockwool granule filter. The oomycete Pythium oligandrum and the bacterium Bacillus subtilis were used as model organisms because of their easy culture, their lack of pathogenicity and their similarity with common oomycete and bacterial plant pathogens. Using real time PCR and species specific oligonueleotides showed that ultrafiltration is highly effective in removing P. oligandrum and B. subtilis. No P. oligandrum or B. subtilis were detected by real time PCR in filtrated nutrient solutions. Passage of nutrient solution through the slow sand filters also resulted in complete removal of P. oligandrum but was less effective for B. subtilis removal.
机译：大多数温室作物都在水培系统中的人工基质上生长。循环养分系统为控制从大棚浸出到环境中的养分溶液提供了一种有利的替代方法，这代表了温室生产区的环境问题。然而，根疾病和其他疾病迅速传播的潜力是温室产业普遍采用再循环养分系统的主要障碍。在这项研究中，使用选择性培养基和直接可行的计数监测了再循环营养液中存在的细菌，真菌和卵菌总数。这三个社区的126位主要代表的序列分析显示，对于真细菌而言，存在11种y变形杆菌，3种Firmiconutes，一种放线菌属和一种黄杆菌属。对于真菌，回收的菌株属于根霉属和Postia。二十个菌株属于超球菌谱系，其中十九个属于腐霉菌属（Oomycota），一个属于斜盘孢菌属（Phaeophyceae）。此外，通过使用切向超滤系统，慢速砂滤器和岩棉颗粒过滤器，评估了超滤和生物过滤消除人工添加到循环营养液中的卵菌和细菌病原体的功效。卵菌腐霉和枯草芽孢杆菌由于其易于培养，缺乏致病性以及与常见卵菌和细菌性植物病原体的相似性而被用作模型生物。使用实时PCR和物种特异性寡核苷酸显示超滤在去除寡聚毕赤酵母和枯草芽孢杆菌中非常有效。通过实时PCR在滤出的营养液中未检测到寡头疟原虫或枯草芽孢杆菌。营养液通过慢速砂滤器也可以完全去除寡头假单胞菌，但对枯草芽孢杆菌的去除效果较差。

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