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Application of Fourier transform infrared ellipsometry to assess the concentration of biological molecules

机译：傅里叶变换红外椭圆仪在评估生物分子浓度中的应用

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Spectroscopic ellipsometry is a noninvasive optical characterization technique mainly used in the semiconductor field to characterize bare substrates and thin films. In particular, it allows the gathering of information concerning the physical structure of the sample, such as roughness and film thickness, as well as its optical response. In the mid-infrared (IR) range each molecule exhibits a characteristic absorption fingerprint,,Which makes this technique chemically selective. Phase-modulated IR ellipsometry does not require a baseline correction procedure or suppression of atmospheric CO2 and water-vapor absorption bands, thus greatly reducing the subjectivity in data analysis. We have found that ellipsometric measurements of thin films, such as the solid residuals left on a plane surface after evaporation of a liquid drop containing a given compound in solution, are particularly favorable for dosing purposes because the intensity of IR absorptions shows a linear behavior along a wide range of solution concentrations of the given compound. Our aim is to illustrate with a concrete example and to justify theoretically the linearity experimentally found between radiation absorption and molecule concentration. For the example, we prepared aqueous solutions of glycogen, a molecule of huge biological importance currently tested in biochemical analyses, at concentrations ranging from 1 mg/l to 1 g/l, which correspond to those found in physiological conditions. The results of this example are promising for the application of ellipsometry for dosing purposes in biochemistry and biomedicine. (C) 2002 Optical Society of America. [References: 20]
机译：椭圆偏振光谱法是一种非侵入性的光学表征技术，主要用于半导体领域以表征裸露的基板和薄膜。特别是，它允许收集有关样品物理结构的信息，例如粗糙度和膜厚以及其光学响应。在中红外（IR）范围内，每个分子均表现出特征性的吸收指纹，这使该技术具有化学选择性。相位调制红外椭圆仪不需要基线校正程序或大气CO2和水汽吸收带的抑制，因此大大降低了数据分析的主观性。我们已经发现，薄膜的椭偏测量，例如在溶液中蒸发包含给定化合物的液滴后残留在平面上的固体残留物，对于定量给药特别有利，因为IR吸收的强度沿线性方向呈线性变化。给定化合物的溶液浓度范围很广。我们的目的是通过一个具体的例子进行说明，并从理论上证明在辐射吸收和分子浓度之间实验得出的线性。例如，我们制备了糖原水溶液，该糖原是一种目前在生化分析中具有重要生物学意义的分子，浓度范围为1 mg / l至1 g / l，与生理条件下的浓度相对应。该实施例的结果有望用于椭圆偏振法在生物化学和生物医学中的定量给药。 （C）2002年美国眼镜学会。 [参考：20]

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