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Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus

机译:荧光相关光谱:起始,生物物理实验和说明书

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摘要

Fluorescence correlation spectroscopy examines the chemical and the photophysical dynamics of dilute molecular solutions by measurement of the dynamic optical fluctuations of the fluorescence of a few molecules, even averaging less than one molecule at a time, in open focal volumes that are usually less than a femtoliter (<10~(-18) m~(3)). It applies the same principles of statistical thermodynamics as does quasi-elastic light scattering. Molecular interactions, conformational changes, chemical reactions, and photophysical dynamics that are not ordinarily detectable by quasi-elastic light scattering can be analyzed by fluorescence correlation spectroscopy in cases in which molecular fluorescence changes in the dynamic range 10~(-7)-10~(2) s.
机译:荧光相关光谱法通过测量通常小于飞升的开放焦点体积中的几个分子(甚至一次平均少于一个分子)的荧光的动态光学波动来检测稀分子溶液的化学和光物理动力学。 (<10〜(-18)m〜(3))。它采用与准弹性光散射相同的统计热力学原理。分子荧光在动态范围10〜(-7)-10〜范围内变化的情况下,可以通过荧光相关光谱法分析准弹性光散射无法检测到的分子相互作用,构象变化,化学反应和光物理动力学。 (2)。

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