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Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control

机译:带有光学反馈控制的水中沙门氏菌鞭毛细丝的近场光学成像

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We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water. #1997 Optical Society of America
机译:我们报告了在光学反馈控制下操作的收集模式近场光学显微镜(c模式NOM)对水中生物样品的高分辨率观察。据我们所知,首次将e逝场功率迅速减小作为反馈信号,就获得了水中沙门氏菌的直型鞭毛细丝图像。单个灯丝的估计直径约为55 nm,像素大小为10 nm。通过在高真空下通过电子显微镜观察获得的标称值25 nm的比较证实,我们的c型NOM在水中可以进行高分辨率成像。 #1997美国眼镜学会

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