• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain cell biology >Imaging synaptic inhibition throughout the brain via genetically targeted Clomeleon.
【24h】

Imaging synaptic inhibition throughout the brain via genetically targeted Clomeleon.

机译：通过基因靶向的Clomeleon对整个大脑的突触抑制进行成像。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Here we survey a molecular genetic approach for imaging synaptic inhibition. This approach is based on measuring intracellular chloride concentration ([Cl(-)](i)) with the fluorescent chloride indicator protein, Clomeleon. We first describe several different ways to express Clomeleon in selected populations of neurons in the mouse brain. These methods include targeted viral gene transfer, conditional expression controlled by Cre recombination, and transgenesis based on the neuron-specific promoter, thy1. Next, we evaluate the feasibility of using different lines of thy1::Clomeleon transgenic mice to image synaptic inhibition in several different brain regions: the hippocampus, the deep cerebellar nuclei (DCN), the basolateral nucleus of the amygdala, and the superior colliculus (SC). Activation of hippocampal interneurons caused [Cl(-)](i) to rise transiently in individual postsynaptic CA1 pyramidal neurons. [Cl(-)](i) increased linearly with the number of electrical stimuli in a train, with peak changes as large as 4 mM. These responses were largely mediated by GABA receptors because they were blocked by antagonists of GABA receptors, such as GABAzine and bicuculline. Similar responses to synaptic activity were observed in DCN neurons, amygdalar principal cells, and collicular premotor neurons. However, in contrast to the hippocampus, the responses in these three regions were largely insensitive to antagonists of inhibitory neurotransmitter receptors. This indicates that synaptic activity can also cause Cl(-) influx through alternate pathways that remain to be identified. We conclude that Clomeleon imaging permits non-invasive, spatiotemporally precise recordings of [Cl(-)](i) in a large variety of neurons, and provides new opportunities for imaging synaptic inhibition and other forms of neuronal chloride signaling.
机译：在这里，我们调查成像突触抑制的分子遗传方法。该方法基于用荧光氯化物指示剂Clomeleon测量细胞内氯化物浓度（[Cl（-）]（i））。我们首先描述几种不同的方式来在小鼠大脑中选定的神经元群体中表达Clomeleon。这些方法包括靶向病毒基因转移，由Cre重组控制的条件表达以及基于神经元特异性启动子thy1的转基因。接下来，我们评估使用不同品系的thy1 :: Clomeleon转基因小鼠在几个不同的大脑区域成像突触抑制的可行性：海马，深小脑核（DCN），杏仁核的基底外侧核和上丘（ SC）。海马interneurons的激活导致[Cl（-）]（i）在个别突触后CA1锥体神经元中短暂上升。 [Cl（-）]（i）随着火车中电刺激的数量线性增加，峰值变化高达4 mM。这些反应很大程度上由GABA受体介导，因为它们被GABA受体和拮抗剂（例如GABAzine）阻断了。在DCN神经元，杏仁核原代细胞和前运动神经元中也观察到类似的对突触活动的反应。但是，与海马相反，这三个区域的反应对抑制性神经递质受体的拮抗剂不敏感。这表明突触活动也可以通过尚待确定的替代途径引起Cl（-）流入。我们得出的结论是，Clomeleon成像允许在多种神经元中无创，时空精确地记录[Cl（-）]（i），并为突触抑制和其他形式的神经元氯信号传递成像提供了新机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号