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首页> 外文期刊>Archives of microbiology >Genome-wide transcriptional changes during the lag phase of Saccharomyces cerevisiae.
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Genome-wide transcriptional changes during the lag phase of Saccharomyces cerevisiae.

机译：酿酒酵母的迟滞期全基因组转录变化。

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摘要

The set of physiological and metabolic changes occurring immediately after inoculation and during the lag phase is thought to be of vital importance for optimal offset of fermentation. The transcriptional changes taking place during the lag phase after inoculation of a late-respiratory-phase yeast culture into fresh, minimal medium were investigated by use of Yeast GeneFilters. In response to the nutritional up-shift, 240 open reading frames were at least five-fold induced and 122 were at least five-fold repressed. These genes were hierarchically clustered according to their lag-phase expression patterns. The majority of the induced genes were most highly induced early in the lag phase, whereas strong repression generally occurred later. Clustering of the genes showed that many genes with similar roles had similar expression patterns. Repressed genes, however, did not cluster as tightly according to function as induced genes. Genes involved in RNA and protein synthesis and processing showed a peak in expression early in the lag phase, except most ribosomal protein genes, which were induced early and whose expression was sustained. Genes involved in chromatin/chromosome structure showed late induction. The correlation between function and expression pattern for these genes indicates regulation by similar mechanisms. Much of the transcriptional response observed appeared to be due to the presence of glucose in the new medium.
机译：接种后立即和滞后阶段发生的一组生理和代谢变化被认为对发酵的最佳补偿至关重要。使用酵母基因过滤器研究了将呼吸后期酵母培养物接种到新鲜的基本培养基中后在迟滞期发生的转录变化。响应于营养上调，诱导了240个开放阅读框至少五倍，而122个被至少抑制五倍。这些基因根据它们的滞后阶段表达模式进行分层聚类。大多数诱导基因在滞后期早期被高度诱导，而强抑制通常发生在后期。基因的聚类表明，许多具有相似作用的基因具有相似的表达模式。然而，受抑制的基因在功能上没有像诱导基因那么紧密地聚集。除了大多数核糖体蛋白基因被早期诱导并持续表达外，参与RNA和蛋白质合成与加工的基因在迟滞期早期表达高峰。涉及染色质/染色体结构的基因显示出晚期诱导。这些基因的功能和表达模式之间的相关性表明通过相似的机制进行调节。观察到的许多转录反应似乎是由于新培养基中存在葡萄糖。

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