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首页> 外文期刊>Archives of microbiology >ANAEROBIC DEGRADATION OF M-CRESOL VIA METHYL OXIDATION TO 3-HYDROXYBENZOATE BY A DENITRIFYING BACTERIUM
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ANAEROBIC DEGRADATION OF M-CRESOL VIA METHYL OXIDATION TO 3-HYDROXYBENZOATE BY A DENITRIFYING BACTERIUM

机译：脱硝菌厌氧降解甲基丙烯酸甲酚氧化为3-羟基苯甲酸酯

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摘要

The anaerobic degradation of m-cresol was studied in a denitrifying bacterium, In the initial studies, hypothetical intermediates of m-cresol degradation were tested in growth experiments and in adaptation studies with dense cell suspensions. Results suggested a degradation of m-cresol via 3-hydroxybenzoate. To verify this, the degradation of m-cresol was followed in concentrated cell suspensions in the presence of metabolic inhibitors. Fluoroacetate treatment resulted in the transient accumulation of substantial amounts of 3-hydroxybenzoate. In the presence of iodoacetamide, not only was 3-hydroxybenzoate transiently formed, but benzoate was also accumulated. These findings support a degradation of nz-cresol via initial anaerobic methyl oxidation to 3-hydroxybenzoate, followed by reductive dehydroxylation to benzoate or benzoyl-CoA. Studies with extracts of m-cresol-grown cells showed the presence of several enzyme activities to be postulated for this pathway. No evidence was found for a carboxylation, hydroxylation of the aromatic ring, or direct ring reduction as the initial step in m-cresol metabolism. [References: 34]
机译：在反硝化细菌中研究了间甲酚的厌氧降解。在最初的研究中，在生长实验和稠密细胞悬浮液的适应性研究中测试了间甲酚降解的假设中间体。结果表明间甲酚通过3-羟基苯甲酸酯降解。为了证实这一点，在代谢抑制剂存在下，在浓缩的细胞悬浮液中追踪间甲酚的降解。氟乙酸盐处理导致大量3-羟基苯甲酸酯的瞬时积累。在碘乙酰胺的存在下，不仅瞬时形成了3-羟基苯甲酸酯，而且还积累了苯甲酸酯。这些发现支持通过最初的厌氧甲基氧化将3-甲酚降解为3-羟基苯甲酸酯，然后还原脱羟基为苯甲酸酯或苯甲酰基-CoA。用间甲酚生长的细胞提取物进行的研究表明，该途径可能存在几种酶活性。在间甲酚代谢的初始步骤中，没有发现羧基化，芳环羟基化或直接环还原的证据。 [参考：34]

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