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High genetic and physiological diversity of sulfate-reducing bacteria isolated from an oligotrophic lake sediment

机译：从贫营养湖泊沉积物中分离的硫酸盐还原细菌的高度遗传和生理多样性

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摘要

The community structure of sulfate-reducing bacteria in littoral and profundal sediments of the oligotrophic Lake Stechlin (Germany) was investigated. A collection of 32 strains was isolated from the highest positive dilutions of most-probable-number series, and their partial 16S rRNA gene sequences and genomic fingerprints based on ERIC (enterobacterial repetitive intergenic consensus)-PCR were analyzed. The strains fell into eight distinct phylogenetic lineages, and the majority (70%) showed a close affiliation to the genus Desulfovibrio. Most of the remaining strains (22%) were related to the gram-positive Sporomusa and Desulfotomaculum groups. A high redundancy of 16S rRNA gene sequences was found within several of the phylogenetic lineages. This low phylogenetic diversity was most pronounced for the subset of strains isolated from oxic sediment layers. ERIC-PCR revealed that most of the strains with identical 16S rRNA gene sequences were genetically different. Since strains with identical 16S rRNA gene sequences but different genomic fingerprints also differed considerably with respect to their physiological capabilities, the high diversity detected in the present work is very likely of ecological relevance. Our results indicate that a high diversity of sulfate-reducing bacterial strains can be recovered from the natural environment using the established cultivation media. [References: 43]
机译：研究了贫营养型斯泰克林湖（德国）沿海和深部沉积物中硫酸盐还原细菌的群落结构。从最高可能数系列的最高阳性稀释液中分离出32个菌株，并分析了它们的部分16S rRNA基因序列和基于ERIC（细菌重复性基因间共识）-PCR的基因组指纹。该菌株分为八个不同的系统发育谱系，大多数（70％）显示与Desulfovibrio属密切相关。其余大多数菌株（22％）与革兰氏阳性孢子菌和Desulfotomaculum组有关。在多个系统发育谱系中发现了高度冗余的16S rRNA基因序列。这种低的系统发育多样性对于从含氧沉积物层分离出的菌株子集最为明显。 ERIC-PCR显示，大多数具有相同16S rRNA基因序列的菌株在遗传上是不同的。由于具有相同16S rRNA基因序列但具有不同基因组指纹的菌株的生理能力也相差很大，因此本研究中检测到的高度多样性很可能具有生态意义。我们的结果表明，使用建立的培养基可以从自然环境中回收高度减少硫酸盐的细菌菌株。 [参考：43]

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