• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of microbiology >A novel short anionic antibacterial peptide isolated from the skin of Xenopus laevis with broad antibacterial activity and inhibitory activity against breast cancer cell
【24h】

A novel short anionic antibacterial peptide isolated from the skin of Xenopus laevis with broad antibacterial activity and inhibitory activity against breast cancer cell

机译：从非洲爪蟾皮肤分离的新型短阴离子抗菌肽，具有广泛的抗菌活性和对乳腺癌细胞的抑制活性

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A vastarray of bioactive peptides from amphibian skin secretions is attracting increasing attention due to the growing problem of bacteria resistant to conventional antibiotics. In this report, a small molecular antibacterial peptide, named Xenopus laevis antibacterial peptide-P1 (XLAsp-P1), was isolated from the skin of Xenopus laevis using reversed-phase high-performance liquid chromatography. The primary structure of XLAsp-P1, which has been proved to be a novel peptide by BLAST search in AMP database, was DEDDD with a molecular weight of 607.7 Da analysed by Edman degradation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). The highlight of XLAsp-P1 is the strong in vitro potency against a variety of Gram-positive and Gram-negative bacteria with minimum inhibitory concentrations (MICs) starting at 10 mu g/mL and potent inhibitory activity against breast cancer cell at tested concentrations from 5 to 50 mu g/mL. In addition, only 6.2 % of red blood cells was haemolytic when incubated with 64 mu g/mL (higher than MICs of all bacterial strain) of XLAsp-P1. The antimicrobial mechanism for this novel peptide was the destruction of the cell membrane investigated by transmission electron microscopy. All these showed that XLAsp-P1 is a novel short anionic antibacterial peptide with broad antibacterial activity and inhibitory activity against breast cancer cell.
机译：由于对常规抗生素具有抗性的细菌日益严重的问题，两栖动物皮肤分泌物中大量的生物活性肽正引起越来越多的关注。在此报告中，使用反相高效液相色谱法从非洲爪蟾的皮肤中分离出了一种名为Xenopus laevis抗菌肽-P1（XLAsp-P1）的小分子抗菌肽。通过AMP数据库中的BLAST搜索已证明XLAsp-P1的主要结构是一种新型肽，通过埃德曼降解和基质辅助激光解吸/电离飞行时间分析了分子量为607.7 Da的DEDDD质谱（MALDI-TOF / TOF-MS）。 XLAsp-P1的亮点是对多种革兰氏阳性和革兰氏阴性细菌具有很强的体外效力，最低抑菌浓度（MICs）从10μg/ mL开始，并且在测试浓度下对乳腺癌细胞具有强抑制活性5至50μg / mL。此外，当与64μg / mL（高于所有细菌菌株的MIC）一起孵育时，只有6.2％的红细胞具有溶血作用。这种新型肽的抗菌机制是通过透射电子显微镜研究破坏细胞膜。所有这些表明XLAsp-P1是一种新型的短阴离子抗菌肽，具有广泛的抗菌活性和对乳腺癌细胞的抑制活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号