The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917

Yang Ying; Yang Yi; Ou Bingming; Xia Pengpeng; Zhou Mingxu; Li Luan; Zhu Guoqiang

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The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917

机译：鞭毛蛋白高变区是益生菌大肠杆菌Nissle 1917菌株中潜在的鞭毛展示域

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The most studied probiotic, Escherichia coli strain Nissle 1917 (EcN) possesses flagella of serotype H1. To explore the potential to use EcN flagellin in flagella display applications, we investigated the effect of deleting amino acids in the hypervariable region of flagellin on EcNc (EcN cured of its two cryptic plasmids pMUT1 and pMUT2). Two EcNc flagellin isogenic mutants with deletions of amino acid residual from 277 to 286 and from 287 to 296 in the hypervariable domain were constructed. Both mutants were flagellated, adherent to IPEC-J2 cells, and colonized BALB/c mice. These hypervariable regions may have future utility in the display of heterologous epitopes.

机译：研究最多的益生菌大肠杆菌菌株Nissle 1917（EcN）具有H1血清型鞭毛。为了探索在鞭毛展示应用中使用EcN鞭毛蛋白的潜力，我们研究了删除鞭毛蛋白高变区中的氨基酸对EcNc（EcN的两个隐性质粒pMUT1和pMUT2固化）的影响。构建了两个EcNc鞭毛蛋白同基因突变体，在高变域中具有277至286和287至296的氨基酸残基缺失。两种突变体都是鞭毛的，附着于IPEC-J2细胞，并定殖于BALB / c小鼠。这些高变区可能在展示异源表位方面具有未来的实用性。

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《Archives of microbiology》 |2016年第7期|共8页
作者
Yang Ying; Yang Yi; Ou Bingming; Xia Pengpeng; Zhou Mingxu; Li Luan; Zhu Guoqiang;
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正文语种 eng
中图分类微生物学;
关键词
E. coli strain Nissle 1917; Flagella display application; Flagellin;

机译：大肠杆菌Nissle菌株1917;Flagella展示应用;Flagellin;

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1. The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917 [J] . Yang Ying, Yang Yi, Ou Bingming, Archives of microbiology . 2016,第7期

机译：鞭毛蛋白高变区是益生菌大肠杆菌Nissle 1917菌株中潜在的鞭毛展示域
2. Escherichia coli strain Nissle 1917-from bench to bedside and back: history of a special Escherichia coli strain with probiotic properties [J] . Sonnenborn Ulrich FEMS Microbiology Letters . 2016,第19期

机译：大肠杆菌菌株Nissle 1917-从工作台到床头和返回：益生菌特殊大肠杆菌菌株的历史
3. Genomic peculiarity of coding sequences and metabolic potential of probiotic Escherichia coli strain Nissle 1917 inferred from raw genome data [J] . Sun J, Gunzer F, Westendorf AM, Journal of Biotechnology . 2005,第2期

机译：从原始基因组数据推断出益生菌大肠杆菌Nissle 1917编码序列的基因组特殊性和代谢潜力
4. CRISPR-based curing and analysis of metabolic burden of cryptic plasmids in Escherichia coli Nissle 1917 [C] . Halimatun S.Zainuddin, Yanfen Bai, Thomas J.Mansell AIChE Annual Meeting . 2019

机译：基于CRISPRICHIA COLI NISLE 1917的CRISPRI基固化与神秘质粒代谢负担分析
5. Engineered outer membrane vesicles derived from probiotic Escherichia coli Nissle 1917 as recombinant subunit antigen carriers for the development of pathogen-mimetic vaccines [D] . Rosenthal, Joseph A. 2014

机译：源自益生菌大肠杆菌Nissle 1917的工程化外膜囊泡，作为重组亚基抗原载体用于开发病原体模拟疫苗
6. Role of F1C Fimbriae Flagella and Secreted Bacterial Components in the Inhibitory Effect of Probiotic Escherichia coli Nissle 1917 on Atypical Enteropathogenic E. coli Infection [O] . Sylvia Kleta, Marcel Nordhoff, Karsten Tedin, 2014

机译：F1C菌毛鞭毛和分泌的细菌成分在益生菌大肠杆菌Nissle 1917对非典型肠致病性大肠杆菌感染的抑制作用中的作用
7. Role of F1C Fimbriae, Flagella, and Secreted Bacterial Components in the Inhibitory Effect of Probiotic Escherichia coli Nissle 1917 on Atypical Enteropathogenic E. coli Infection [O] . Sylvia Kleta, Marcel Nordhoff, Karsten Tedin, 2014

机译：F1C FIMBRIAE，鞭毛和分泌细菌组分在益生菌大肠杆菌尼斯1917对非典型肠球菌大肠杆菌感染的抑制作用中的作用
8. Synthesis of Aerobactin and a 76,000-Dalton Iron-Regulated Outer Membrane Protein by Escherichia coli K-12-Shigella flexneri Hybrids and by Enteroinvasive Strains of Escherichia coli [R] . Griffiths, E., Stevenson, P., Hale, T. L., 1985

机译：大肠埃希氏菌K-12-shigella flexneri杂交种和大肠杆菌肠道侵袭菌合成aerobactin和76,000-Dalton铁调节的外膜蛋白

The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917

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