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首页> 外文期刊>Archives of microbiology >Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum
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Indole-3-acetic acid biosynthetic pathways in the basidiomycetous yeast Rhodosporidium paludigenum

机译：担子菌酵母paludigenum中的吲哚-3-乙酸生物合成途径

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摘要

Microorganisms produce plant growth regulators, such as auxins, cytokinins and gibberellins, to promote plant growth. Auxins are a group of compounds with an indole ring that have a positive effect on plant growth. Indole-3-acetic acid (IAA) is a plant growth hormone classified as an indole derivative of the auxin family. IAA biosynthesis pathways have been reported and widely studied in several groups of bacteria. Only a few studies on IAA biosynthesis pathways have been conducted in yeast. This study aimed to investigate IAA biosynthesis pathways in a basidiomycetous yeast (Rhodosporidium paludigenum DMKU-RP301). Investigations were performed both with and without a tryptophan supplement. Indole compound intermediates were detected by gas chromatography-mass spectrometry. Indole-3-lactic acid and indole-3-ethanol were found as a result of the enzymatic reduction of indole-3-pyruvic acid and indole-3-acetaldehyde, in IAA biosynthesis via an indole-3-pyruvic acid pathway. In addition, we also found indole-3-pyruvic acid in culture supernatants determined by high-performance liquid chromatography. Identification of tryptophan aminotransferase activity supports indole-3-pyruvic acid-routed IAA biosynthesis in R. paludigenum DMKU-RP301. We hence concluded that R. paludigenum DMKU-RP301 produces IAA through an indole-3-pyruvic acid pathway.
机译：微生物产生植物生长调节剂，例如植物生长素，细胞分裂素和赤霉素，以促进植物生长。生长素是一组带有吲哚环的化合物，对植物的生长有积极作用。吲哚-3-乙酸（IAA）是一种植物生长激素，被归类为生长素家族的吲哚衍生物。 IAA生物合成途径已被报道并在几类细菌中进行了广泛的研究。在酵母中仅进行了一些有关IAA生物合成途径的研究。这项研究旨在调查在担子菌酵母（Rhodosporidium paludigenum DMKU-RP301）中IAA的生物合成途径。在有和没有色氨酸补充剂的情况下均进行了研究。通过气相色谱-质谱法检测吲哚化合物中间体。在通过吲哚-3-丙酮酸途径进行的IAA生物合成中，吲哚-3-丙酮酸和吲哚-3-乙醛被酶催化还原，结果发现吲哚-3-乳酸和吲哚-3-乙醇。此外，我们还通过高效液相色谱法测定了培养上清液中的吲哚-3-丙酮酸。色氨酸氨基转移酶活性的鉴定支持在paludigenum DMKU-RP301中吲哚-3-丙酮酸路由的IAA生物合成。因此，我们得出结论，paludigenum DMKU-RP301可以通过吲哚-3-丙酮酸途径产生IAA。

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