...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >The effects of methidathion on lipid peroxidation and some liver enzymes: role of vitamins E and C.
【24h】

The effects of methidathion on lipid peroxidation and some liver enzymes: role of vitamins E and C.

机译：甲硫磷对脂质过氧化和某些肝脏酶的影响：维生素E和C的作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Methidathion (MD) [ O, O-dimethyl S-(2,3-dihydro-5-methoxy-2-oxo-1,3,4-thiadiazol-3-ylmethyl) phosphorodithioate] is one of the most widely used organophosphate insecticides (OPIs) in agriculture and public health programmes. We have, therefore, examined the in vivo and in vitro effects of MD on the serum activities of cholinesterase (ChE), enzymes concerning liver damage and lipid peroxidation (LPO; only in vivo), and have evaluated the ameliorating effects of a combination of vitamins E and C against MD toxicity. The in vivo experimental groups were: control group, MD-treated group (MD), and a group treated with MD plus vitamin E plus vitamin C (MD+Vit). The MD and MD+Vit groups were treated orally with a single dose of 8 mg MD/kg body weight at 0 h. Vitamin E and vitamin C were injected at doses of 150 mg/kg body weight i.m. and 200 mg/kg body weight i.p., respectively, 30 min after the treatment with MD in the MD+Vit group. Blood samples were taken 24 h after the MD administration. For in vitro study, venous blood samples were obtained from volunteers, and serum recovered. The activities of serum enzymes were determined in each sample and these served as 0 h values. Each sample was divided into four portions, each of which served as one of the experimental groups, as follows: control group, vitamin E plus vitamin C group (Vit), MD-treated group (MD) and MD plus vitamin E plus vitamin C group (MD+Vit). Vitamin E and vitamin C were added at doses of 7.5 and 10 micro g/ml, respectively, into the Vit and MD+Vit groups. MD was added at doses of 0.4 mg/ml into the MD and MD+Vit groups. The activities of serum enzymes were determined in each sample at 24 h. The results of the in vivo experiment demonstrated that thiobarbituric acid reactive substances were increased in the MD group compared with the control group, and decreased in the MD+Vit group compared with MD group. ChE activity was decreased in both MD and MD+Vit groups compared with controls and increased in the MD+Vit group compared with the MD group. The activities of aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) and lactate dehydrogenase (LDH) were increased in both the MD and MD+Vit groups compared with the control group. AST activity was decreased in MD+Vit group compared with the MD group. Alanine aminotransferase (ALT) activity was decreased in both the MD and MD+Vit groups compared with control group. The results of in vitro experiment showed that all enzyme activities remained unchanged in both the control and Vit groups compared with values at 0 h. The activities of ChE, ALT and LDH were decreased in both the MD and MD+Vit groups compared with 0 h values. There was no significant difference between the MD and MD+Vit groups. The activities of AST, ALP and GGT remained unchanged in all groups. From these results, it can be concluded that MD caused liver damage, and LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Single-dose treatment with a combination of vitamins E and C after the administration of MD can reduce LPO caused by MD.
机译：甲硫磷（MD）[O，O-二甲基S-（2,3-二氢-5-甲氧基-2-氧代-1,3,4-噻二唑-3-基甲基）二硫代磷酸硫酯]是使用最广泛的有机磷杀虫剂之一农业和公共卫生计划中的（OPI）。因此，我们研究了MD对胆碱酯酶（ChE），与肝损伤和脂质过氧化有关的酶（LPO;仅在体内）的血清活性的体内和体外影响，并评估了下列药物组合的改善作用维生素E和C对抗MD毒性。体内实验组为：对照组，MD治疗组（MD）和用MD加维生素E加维生素C治疗的组（MD + Vit）。在0 h，以8 mg MD / kg体重的单剂量口服治疗MD和MD + Vit组。维生素E和维生素C的注射剂量为i.m体重为150 mg / kg。在MD + Vit组中，用MD治疗30分钟后，腹腔内注射剂量分别为200 mg / kg体重和200 mg / kg体重。 MD给药后24小时取血样。为了进行体外研究，从志愿者那里获得静脉血样本，并回收血清。测定每个样品中的血清酶活性，将其作为0小时值。每个样品分为四个部分，分别作为实验组之一，分别为：对照组，维生素E加维生素C组（Vit），MD治疗组（MD）和MD加维生素E加维生素C组（MD + Vit）。分别以7.5和10微克/毫升的剂量将维生素E和维生素C添加到Vit和MD + Vit组中。将MD以0.4mg / ml的剂量加入MD和MD + Vit组。在24小时时测定每个样品中的血清酶活性。体内实验结果表明，与对照组相比，MD组中的硫代巴比妥酸反应性物质增加，而MD + Vit组中的硫代巴比妥酸反应性物质减少。与对照组相比，MD和MD + Vit组的ChE活性均降低，而MD + Vit组的ChE活性则高于MD组。与对照组相比，MD组和MD + Vit组的天冬氨酸转氨酶（AST），碱性磷酸酶（ALP），γ-谷氨酰转移酶（GGT）和乳酸脱氢酶（LDH）的活性均增加。与MD组相比，MD + Vit组的AST活性降低。与对照组相比，MD组和MD + Vit组的丙氨酸氨基转移酶（ALT）活性均降低。体外实验结果表明，与0 h时相比，对照组和Vit组的所有酶活性均保持不变。 MD和MD + Vit组的ChE，ALT和LDH活性均低于0 h。 MD组和MD + Vit组之间没有显着差异。 AST，ALP和GGT的活动在所有组中均保持不变。从这些结果可以得出结论，MD引起肝脏损伤，LPO可能是MD引起毒性的分子机制之一。 MD给药后，维生素E和C的单剂量治疗可减少MD引起的LPO。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号