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首页> 外文期刊>Archives of microbiology >Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments
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Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

机译：依赖文化和不依赖文化的方法揭示了陆地环境中不同的甲基营养群落

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摘要

One-carbon compounds such as methanol, dimethylsulfide (DMS) and dimethylsulfoxide (DMSO) are significant intermediates in biogeochemical cycles. They are suggested to affect atmospheric chemistry and global climate. Methylotrophic microorganisms are considered as a significant sink for these compounds; therefore, we analyzed the diversity of terrestrial bacteria that utilize methanol, DMS and DMSO as carbon and energy source using culture-dependent and culture-independent methods. The effect of habitat type on the methylotrophic community structure was also investigated in rhizosphere and bulk soil. While thirteen strains affiliated to the genera Hyphomicrobium, Methylobacterium, Pseudomonas, Hydrogenophaga, Rhodococcus, Flavobacterium and Variovorax were isolated, denaturing gradient gel electrophoresis revealed the dominance of Thiobacillus, Rhodococcus, Flavobacterium and Bacteroidetes species. Furthermore, methylotrophic communities that degrade methanol or DMS are not shaped by terrestrial habitat type. Rhizosphere and soil samples showed dominance of Methylophilus spp. and Methylovorus spp. for methanol enrichments; Cytophaga spp., Pseudomonas tremae and Thiobacillus thioparus for DMS enrichments.
机译：一碳化合物（例如甲醇，二甲基硫醚（DMS）和二甲基亚砜（DMSO））是生物地球化学循环中的重要中间体。建议它们影响大气化学和全球气候。甲基营养微生物被认为是这些化合物的重要吸收源。因此，我们使用依赖于培养物和不依赖于培养物的方法分析了利用甲醇，DMS和DMSO作为碳和能源的陆生细菌的多样性。还研究了根际和块状土壤中生境类型对甲基营养型群落结构的影响。分离了13株隶属于Hyphomicrobium，Methylobacterium，Pseudomonas，Hydrogenophaga，Rhodococcus，Flavobacterium和Variovorax的菌株，变性梯度凝胶电泳揭示了Thiobacillus，Rhodococcus，Flavobacterium和Bacteroidetes菌的优势。此外，降解甲醇或DMS的甲基营养型群落不受陆地生境类型的影响。根际和土壤样品显示出嗜甲基菌属。和甲基甲虫。用于甲醇浓缩; Cyphaphaga spp。，假单胞菌和硫代硫杆菌（Thiobacillus thioparus）用于DMS富集。

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