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Bacillus cellulasensis sp nov., isolated from marine sediment

机译:分离自海洋沉积物的纤维素芽孢杆菌

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摘要

A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed < 97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C-15:0, anteiso-C-15:0, iso-C-17:0, and anteiso-C-17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816T. On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T) = CCTCC AB 2011126(T)).
机译:从印度果阿省乔罗岛上提取的沉积物样品中分离出一种新型细菌NIO-1130(T),并对其进行了分类研究。该菌株是革兰氏阳性,有氧运动的。根据16S rRNA基因序列进行的系统进化分析将分离株置于芽孢杆菌NIO-1130(T)属中,显示与嗜盐芽孢杆菌DSM 25387(T)(98.4%)和尼亚芽芽孢杆菌CIP 109816(T)的序列相似性最高(98.1% ),而其他芽孢杆菌属菌种的相似度小于97.0%。基于gyrB基因序列的树揭示了菌株杆菌群。主要的甲萘醌为MK-7,主要的细胞脂肪酸为iso-C-15:0,anteiso-C-15:0,iso-C-17:0和anteiso-C-17:0。该菌株显示出39.9mol%的DNA G + C含量。 DNA-DNA杂交研究表明,菌株NIO-1130(T)与嗜盐芽孢杆菌DSM 25387(T)和niacensis Cab 109816T具有70%的相似性。在生理,生化,化学分类和系统发育分析的基础上,我们认为该分离物代表芽孢杆菌属的一种新种,其名称为芽胞杆菌。建议。类型菌株为NIO-1130(T)(= NCIM 5461(T)= CCTCC AB 2011126(T))。

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