...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >A potential role of calcium in apoptosis and aberrant chromatin forms in porcine kidney PK15 cells induced by individual and combined ochratoxin A and citrinin
【24h】

A potential role of calcium in apoptosis and aberrant chromatin forms in porcine kidney PK15 cells induced by individual and combined ochratoxin A and citrinin

机译：钙在单独和联合A曲毒素A和西林蛋白诱导的猪肾PK15细胞凋亡和染色质异常形成中的潜在作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to establish the involvement of calcium signalling in genotoxicity, apoptosis and necrosis evoked by ochratoxin A (OTA) and citrinin (CTN) alone or in combination in porcine kidney PK15 cells. Cell proliferation test (MTT) and trypan blue assays (24 h) demonstrated that CTN (IC 50 = 73.5 ± 1.0, 75.4 ± 1.4 μM, respectively) was less toxic than OTA (IC 50 = 14.0 ± 2.4, 20.5 ± 1.0 μM, respectively). To test their cytotoxic interactions, two doses of single OTA (6 and 10 μM) and CTN (30 and 50 μM) and their combinations were applied. Combined treatment showed additive cytotoxic effects. OTA and CTN induced dose-dependent increase in cytosolic calcium level (assessed with Fura-2 AM). However, combined treatment did not provoke additional increase in calcium signal. The rate of apoptosis and necrosis (DAPI-antifade staining) was significantly higher after 12 h than 24 h, while the frequencies of micronuclei (MNs) and nuclear buds (NBs) were higher after 24 h than 12 h treatment. Combined exposure resulted in apoptotic and necrotic synergism, while genotoxic effects of OTA + CTN were noted as antagonistic or additive. Co-exposure of cells to calcium chelator BAPTA-AM significantly reduced CTN and OTA + CTN-evoked apoptosis. Twenty-four hour after co-exposure to BAPTA-AM and a single OTA and CTN, MNs significantly decreased while NBs dropped significantly after co-treatment with BAPTA-AM and OTA + CTN. In conclusion, disturbance of Ca 2+ homeostasis caused by OTA and CTN plays a significant role in cell genotoxicity and death.
机译：这项研究的目的是建立钙信号传导在猪肾PK15细胞中单独或联合使用曲霉毒素A（OTA）和citrinin（CTN）引起的遗传毒性，细胞凋亡和坏死。细胞增殖试验（MTT）和锥虫蓝试验（24 h）表明，CTN（分别为IC 50 = 73.5±1.0，75.4±1.4μM）的毒性比OTA（IC 50 = 14.0±2.4，20.5±1.0μM，分别）。为了测试它们的细胞毒性相互作用，应用了两种剂量的单一OTA（6和10μM）和CTN（30和50μM）及其组合。联合治疗显示出附加的细胞毒性作用。 OTA和CTN诱导了胞浆钙水平的剂量依赖性增加（用Fura-2 AM评估）。但是，联合治疗并未引起钙信号的进一步增加。处理12 h后，细胞凋亡和坏死率（DAPI染色）显着高于处理24 h，而处理24 h后，微核（MNs）和核芽（NBs）的频率高于处理12 h。联合暴露导致凋亡和坏死的协同作用，而OTA + CTN的遗传毒性作用被认为是拮抗或累加的。细胞与钙螯合剂BAPTA-AM共同暴露可显着降低CTN和OTA + CTN诱发的细胞凋亡。与BAPTA-AM和OTA + CTN共同处理后，与BAPTA-AM和单一OTA和CTN共同暴露24小时后，MNs显着下降，而NBs显着下降。总之，由OTA和CTN引起的Ca 2+稳态紊乱在细胞遗传毒性和死亡中起着重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号