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首页> 外文期刊>Breeding science >Assessment of genetic diversity of Philippine rice cultivars carrying good quality traits using SSR markers.
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Assessment of genetic diversity of Philippine rice cultivars carrying good quality traits using SSR markers.

机译：使用SSR标记评估具有良好品质特性的菲律宾水稻品种的遗传多样性。

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Microsatellite markers, also known as simple sequence repeat (SSR) markers have been useful for the detection of genetic diversity. Twenty-four rice cultivars carrying good quality traits were evaluated for genetic diversity using 164 SSR markers. A total of 890 alleles were detected by 151 polymorphic markers with an average of 5.89 per locus. Out of these markers, 89 generated a total of 147 rare alleles. Based on Shannon's diversity index, an overall genetic diversity of 0.71 was revealed indicating a high level of genetic variation among these cultivars. Polymorphism information content (PIC) values of the markers ranged from 0.18 (RM420) to 0.91 (RM473B) with an average of 0.68 per marker. Cluster analysis of these cultivars enabled to identify 3 groups at 40% level of similarity with additional sub-clusters within each group. Group 1 corresponded to the 8 japonica subspecies, whereas Groups 2 and 3 comprised the indica. Cultivars under groups 1 and 2 are known for their aroma and good cooking and eating quality traits. Between the 2 rice subspecies, indica gave more alleles than japonica and likewise displayed a higher genetic diversity. Genetic diversity of indica was high on chromosome 11, while that for japonica was high on chromosome 2. The study revealed that SSR markers facilitated the classification of these cultivars according to their subspecies. The results also indicated that these quality rice cultivars exhibited a higher genetic diversity and therefore very useful for rice breeding programs, especially for genetic mapping studies and eventually for application of marker-assisted selection (MAS) in the programs.
机译：微卫星标记（也称为简单序列重复（SSR）标记）已用于检测遗传多样性。使用164个SSR标记对24个具有优良品质性状的水稻品种进行了遗传多样性评估。通过151个多态性标记检测到890个等位基因，平均每个位点为5.89。在这些标记中，有89个产生了147个稀有等位基因。根据香农的多样性指数，总体遗传多样性为0.71，表明这些品种之间的遗传变异水平很高。标记的多态信息含量（PIC）值介于0.18（RM420）至0.91（RM473B）之间，每个标记平均0.68。通过对这些品种的聚类分析，可以确定3个组，相似度为40％，并且每个组中都有其他子组。第1组对应于8个粳稻亚种，而第2和第3组包含the稻。第1组和第2组的品种以其香气，良好的烹饪和饮食品质特征而闻名。在这两个水稻亚种之间，in稻的等位基因比粳稻多，并且遗传多样性也更高。 d稻的遗传多样性在11号染色体上较高，而粳稻的遗传多样性在2号染色体上较高。研究表明，SSR标记有助于根据其亚种对这些品种进行分类。结果还表明，这些优质的水稻品种显示出更高的遗传多样性，因此对于水稻育种计划特别是对于遗传图谱研究以及最终在该计划中应用标记辅助选择（MAS）非常有用。

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